Molecular structure of an N-formyltransferase from Providencia alcalifaciens O30

Protein Science : a Publication of the Protein Society
Nicholas A GentheH M Holden

Abstract

The existence of N-formylated sugars in the O-antigens of Gram-negative bacteria has been known since the middle 1980s, but only recently have the biosynthetic pathways for their production been reported. In these pathways, glucose-1-phosphate is first activated by attachment to a dTMP moiety. This step is followed by a dehydration reaction and an amination. The last step in these pathways is catalyzed by N-formyltransferases that utilize N(10) -formyltetrahydrofolate as the carbon source. Here we describe the three-dimensional structure of one of these N-formyltransferases, namely VioF from Providencia alcalifaciens O30. Specifically, this enzyme catalyzes the conversion of dTDP-4-amino-4,6-dideoxyglucose (dTDP-Qui4N) to dTDP-4,6-dideoxy-4-formamido-d-glucose (dTDP-Qui4NFo). For this analysis, the structure of VioF was solved to 1.9 Å resolution in both its apoform and in complex with tetrahydrofolate and dTDP-Qui4N. The crystals used in the investigation belonged to the space group R32 and demonstrated reticular merohedral twinning. The overall catalytic core of the VioF subunit is characterized by a six stranded mixed β-sheet flanked on one side by three α-helices and on the other side by mostly random coil. This N-terminal ...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R J AlmassyZ Hostomska
Jun 20, 1993·Journal of Molecular Biology·R A LaskowskiJ M Thornton
Sep 28, 2001·The Journal of Infectious Diseases·T MurataT Honda
Oct 24, 2003·Acta Crystallographica. Section D, Biological Crystallography·Simon Parsons
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Nov 24, 2004·Southern Medical Journal·Patrick R Krake, Neeraj Tandon
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Feb 14, 2006·Carbohydrate Research·Nina A KocharovaAntoni Rozalski
Apr 30, 2009·Molecular Plant Pathology·Teresa A Coutinho, Stephanus N Venter
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Oct 29, 2010·Journal of Clinical Microbiology·Oguz Resat SipahiSercan Ulusoy
Aug 1, 2013·Biochemistry·James B ThodenHazel M Holden
Sep 10, 2013·Biochemistry. Biokhimii︠a︡·O G OvchinnikovaY A Knirel
Dec 19, 2013·Protein Science : a Publication of the Protein Society·Alex L ZimmerHazel M Holden

❮ Previous
Next ❯

Citations

Mar 7, 2017·Journal of Structural Biology·Colin R WoodfordHazel M Holden
May 16, 2018·Protein Science : a Publication of the Protein Society·Haley A BrownHazel M Holden
Jan 23, 2019·Protein Science : a Publication of the Protein Society·Daniel L HofmeisterHazel M Holden
Jun 21, 2019·The American Journal of Tropical Medicine and Hygiene·Mohammad Monir ShahYoshio Ichinose
Dec 24, 2019·Protein Science : a Publication of the Protein Society·Nicholas M GirardiHazel M Holden
Feb 23, 2020·Protein Science : a Publication of the Protein Society·Justin D FerekHazel M Holden
May 14, 2016·Protein Science : a Publication of the Protein Society·Nicholas A GentheHazel M Holden
Jan 29, 2019·Archives of Biochemistry and Biophysics·Nikola KenjićAudrey L Lamb
Jul 1, 2017·Biochemistry·Murray M DunsirnHazel M Holden
Jun 22, 2017·Biochemistry·Alexander S RiegertHazel M Holden
Aug 12, 2021·Protein Science : a Publication of the Protein Society·Colton J HeisdorfHazel M Holden

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.