PMID: 9193005May 30, 1997Paper

Molecular structure of the lipoamide dehydrogenase domain of a surface antigen from Neisseria meningitidis

Journal of Molecular Biology
I Li de la SierraG Padrón

Abstract

The protein p64k from the surface of the Neisseria meningitidis bacteria has been characterized as a two-domain protein. It contains a dihydrolipoamide dehydrogenase domain of 482 residues, involving a FAD prosthetic group as a cofactor, and a smaller lipoic acid binding domain of 86 residues. The two domains are joined by a flexible segment rich in alanine and proline residues. The structure of the dihydrolipoamide dehydrogenase domain was determined by X-ray diffraction. It was solved by a combination of molecular replacement and multiple isomorphous replacement techniques and refined to 2.7 A resolution. In the crystal, the recombinant p64k mimics the functional homo-dimer by using one of the crystallographic 2-fold axes. The reactive disulphide bridge Cys161-Cys166 is in the oxidised state and the FAD is bound in an extended conformation. This main domain contains the major antigenic determinant of the protein, an extended loop of 32 residues at the surface of the protein. A mis-attribution at residue 553 in the sequence has been detected by inspection of electron density maps and the geometry. However, when compared to the other dihydrolipoamide dehydrogenases, there are some significant differences: (1) an unusual number ...Continue Reading

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Citations

Nov 5, 1999·Protein Science : a Publication of the Protein Society·N NaganoJ M Thornton
May 5, 2005·Canadian Journal of Physiology and Pharmacology·André M CantinBenoit Paquette
Aug 11, 2007·The Journal of Biological Chemistry·Anders P Håkansson, Alexander W Smith
Aug 10, 2000·Scandinavian Journal of Immunology·S GonzálezR Silva
Mar 24, 2006·FEMS Immunology and Medical Microbiology·Antonio E PérezGerardo Guillén
Sep 15, 2004·FEMS Immunology and Medical Microbiology·Sonia GonzálezRicardo Silva
Mar 13, 2003·European Journal of Biochemistry·Victoria I Bunik
Jul 10, 1998·Biochimica Et Biophysica Acta·A de KokA H Westphal
Jun 25, 2021·Journal of Molecular Recognition : JMR·Gideon Fleminger, Avraham Dayan

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