PMID: 3765825Aug 1, 1986Paper

Molecular studies of the differential replication at pyrexial temperatures of two influenza viruses differing in virulence for ferrets

Virus Research
H A OvertonH Smith

Abstract

Replication of a virulent clone (7a) of the reassortant influenza virus A/Puerto Rico/8/34-A/England/939/69 (H3N2) in ferret nasal turbinate tissue is less affected than that of an attenuated clone (64d) by temperatures which occur during pyrexia in ferrets. This is a factor which contributes to the difference in virulence of the two clones. The differential replication of the two clones at pyrexial temperatures has been reproduced in allantois-on-shell (egg-bit) cultures, and the synthesis of viral polypeptides and RNA species examined. This virus-host system was chosen because it was more convenient to use than organ cultures but, like the latter, might provide information relevant to the in vivo situation. With this system it was not possible to achieve single cycle replication: the observed effects are cumulative over several (2 to 3) cycles of replication (24 h) and therefore conclusions from them may not be as definitive as those from single cycle conditions. However, in cells infected with clone 64d both A(+) cRNA and polypeptide synthesis were little affected at 40 degrees C but levels were decreased by about 70-80% at 41 degrees C; A(+) cRNA and polypeptide levels were unaffected even at 41 degrees C with clone 7a. The...Continue Reading

References

Sep 6, 1976·Biochimica Et Biophysica Acta·J M TaylorJ Summers
Oct 15, 1979·Virology·T BarrettB W Mahy
Nov 1, 1978·Virology·R A LambP W Choppin
Dec 1, 1977·Virology·A J HayJ J Skehel
Jul 15, 1976·Virology·M B Richey, P Palese
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Mar 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·V Paffenholz, C Scholtissek
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
May 1, 1983·The Biochemical Journal·J W McCauley, B W Mahy
Mar 1, 1984·The Journal of General Virology·S G Markushin, Y Z Ghendon
Apr 15, 1982·Virology·G L Smith, A J Hay
Jan 1, 1979·Perspectives in Biology and Medicine·R M Chanock, B R Murphy

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Citations

Apr 24, 2013·Journal of Theoretical Biology·Lisa N MurilloAlan S Perelson
Oct 1, 2004·Lab Animal·John A Maher, Joanne DeStefano
Feb 2, 2021·World Journal of Clinical Cases·Lucas BelonEdward Walter

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