Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR

Journal of Clinical Microbiology
Gregory R SiragusaS E Craven

Abstract

Clostridium perfringens strains (type A) isolated from an integrated poultry operation were subtyped using repetitive-element PCR with Dt primers. Isolates were obtained from fecal, egg shell, fluff, and carcass rinse samples as part of a previously reported temporally linked epidemiological survey. A total of 48 isolates of C. perfringens were obtained from different stages of the broiler chicken production chain from two separate breeder farms that supplied a single hatchery that in turn provided chicks to a single grow-out farm whose flocks were processed at a single plant. All 48 isolates were typeable (100% typeability) by repetitive-element PCR with Dt primers. This subtyping method was highly reproducible and discriminatory. By repetitive-element PCR with Dt primers, isolates were classified into four major branches with 12 subgroups or clades. The Simpson's index of discrimination was calculated to be 0.96 for groupings of >95% correlation. Toxin gene profiles of the isolates indicated that all of the isolates were C. perfringens alpha-toxin gene positive and 46 of 48 isolates were beta2-toxin gene positive. All strains were negative for beta- and epsilon-toxin genes. Repetitive sequence-based PCR was found to be a tech...Continue Reading

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Citations

Aug 14, 2010·Avian Pathology : Journal of the W.V.P.A·Guangxing LiGregory R Siragusa
Aug 22, 2009·Foodborne Pathogens and Disease·Marin Pavlic, Mansel W Griffiths
Jun 17, 2008·Letters in Applied Microbiology·I ErolG Hildebrandt
Nov 26, 2009·Journal of Applied Microbiology·S R Mueller-SpitzS L McLellan

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