Molecular subtyping scheme for Salmonella panama.

Journal of Clinical Microbiology
J StanleyA P Burnens

Abstract

We describe a genotyping scheme for Salmonella panama. Defined probes specific for the 16S rRNA gene and the DNA insertion element IS200 were generated by PCR from S. panama and were used to probe genomic Southern blots made with enzymes selected to cut within and outside the probed sequences. Plasmid profiles were determined. The typeability and discriminatory power of the individual methods were compared. Ribotyping with 16S rRNA gene probe alone was slightly more discriminatory than phage typing, but unlike the latter, ribotyping was able to type all strains. IS200 profiling was the single most discriminatory method for S. panama, having an index of discrimination (D) of 0.8 and 100% typeability. Plasmid profiling, which had moderate discriminatory power but only 50% typeability, was valuable as an adjunct technique. The use of all three methods together or simply the combination of IS200 profiling with the two most discriminatory enzymes and plasmid profiling yielded a molecular typing scheme whose discriminatory power (D = 0.97) approached the maximum theoretical value. This should prove both useful and robust for epidemiological investigations of S. panama.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J BrosiusH F Noller
Apr 5, 1975·The Veterinary Record·B M Williams
Oct 1, 1992·Acta Paediatrica·A A KostialaM Muttilainen
Jan 1, 1989·British Journal of Obstetrics and Gynaecology·A A Kostiala, T Ranta
Jan 20, 1986·Journal of Molecular Biology·S Lam, J R Roth
Jun 1, 1988·Epidemiology and Infection·E G Wilkins, C Roberts
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Nov 1, 1994·Letters in Applied Microbiology·J R GibsonR J Owen
Aug 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J N MaslowR D Arbeit
Mar 1, 1993·Molecular Microbiology·P A GuligM Rhen

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Citations

Jul 11, 2013·World Journal of Microbiology & Biotechnology·Yousra TurkiHadda Ouzari

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