Molecular surveillance of methicillin-resistant Staphylococcus aureus by multiple-locus variable number tandem repeat fingerprinting (formerly multiple-locus variable number tandem repeat analysis) and spa typing in a hierarchic approach

Diagnostic Microbiology and Infectious Disease
Michal KarynskiWaleria Hryniewicz

Abstract

In this study, clonal relatedness of 202 Staphylococcus aureus (mostly methicillin-resistant) isolates recovered in 29 Polish hospitals was investigated by multiple-locus variable number tandem repeat fingerprinting (MLVF) and spa typing. Our analysis yielded 69 MLVF patterns and 34 spa types. Almost all isolates (97.4%) identical by MLVF were also indistinguishable by spa typing. Therefore, the MLVF method can be a cheap and fast screen before spa typing. Moreover, results obtained by MLVF suggest a set of simple criteria for grouping of spa types. The proposed algorithm groups isolates into the same cluster when spa sequences differ by a single mutation event: i) a single deletion or insertion of repeat unit(s) at the X region of the protein A gene or ii) a single nucleotide polymorphism within a repeat sequence. The combined use of these 2 methods, MLVF in local laboratories and spa typing of selected isolates in reference centers, can improve the monitoring of hospital-to-hospital strain transmission events and public health interventions on a huge scale.

References

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Oct 6, 2006·Journal of Clinical Microbiology·Artur SabatWaleria Hryniewicz

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Citations

Sep 29, 2012·Emerging Infectious Diseases·Shu-Hua WangUNKNOWN Prevention Epicenter Program of the Centers for Disease Control and Prevention
Nov 17, 2012·Journal of Microbiological Methods·Jonathan Hon-Kwan ChenKwok-Yung Yuen
Dec 3, 2014·Diagnostic Microbiology and Infectious Disease·Shu-Hua WangUNKNOWN Prevention Epicenter Program of the Centers for Disease Control and Prevention
Mar 1, 2016·Journal of Infection and Public Health·Farzad KhademiAzad Khaledi
Nov 13, 2010·Journal of Medical Microbiology·Kanokporn MongkolrattanothaiRavindra K Vegunta
Jun 22, 2013·International Journal of Medical Microbiology : IJMM·Monika A ChlebowiczGirbe Buist
Jun 30, 2012·PloS One·J Natalia JiménezMargarita M Correa
Sep 12, 2015·PLoS Neglected Tropical Diseases·Nana Ama AmissahYmkje Stienstra
Jun 16, 2016·Frontiers in Microbiology·Daniela M GeraciUNKNOWN NICU Surveillance Study Group
Aug 11, 2011·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Gopi PatelDavid P Calfee
Aug 17, 2011·The Journal of Infectious Diseases·Juhsien J C NienaberUNKNOWN International Collaboration on Endocarditis-Microbiology Investigators
Jun 22, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Seong-Ho ChoiVance G Fowler

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