Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.

Ticks and Tick-borne Diseases
Eleonor Castro JanerMaría Ernestina Olhagaray

Abstract

The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele-specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4-5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% ...Continue Reading

References

Dec 1, 1994·Revue Scientifique Et Technique·S E Kunz, D H Kemp
Aug 12, 1999·Biochemical and Biophysical Research Communications·H HeJ E George
Sep 11, 2013·Pesticide Biochemistry and Physiology·Frank D RinkevichKe Dong
Apr 15, 2015·Veterinary Parasitology·E Castro JanerT T S Schumaker
Jun 2, 2017·Ticks and Tick-borne Diseases·Luciana Gatto BritoMárcia Cristina de Sena Oliveira
Jun 10, 2017·Pesticide Biochemistry and Physiology·K M U Janadaree Bandara, S H P Parakrama Karunaratne
Sep 21, 2017·Frontiers in Bioscience (Landmark Edition)·Nicholas N JonssonHh Caline Koh-Tan
Mar 20, 2019·International Journal for Parasitology. Drugs and Drug Resistance·Guilherme M KlafkeAdalberto A Pérez de León
Mar 23, 2019·Ticks and Tick-borne Diseases·E Castro JanerT S S Schumaker

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