PMID: 7028097Aug 18, 1981Paper

Molecular symmetry and metastable states of enzymes exhibiting half-of-the-sites reactivity

Biochemistry
J HerzfeldD Jung

Abstract

Half-of-the-sites reactivity in oligomeric enzymes has generally been accepted as evidence for structural asymmetry between subunits. However, we show that the symmetric two-state allosteric model [Monod, J., Wyman, J., & Changeux, J.-P. (1965) J. Mol. Biol. 12, 88-118] is quantitatively consistent with half-of-the-sites reactivity data for several hexameric and tetrameric enzymes. Specifically, the time courses for both the modification and the inactivation of glutamate dehydrogenase by glutamyl alpha-chloromethyl ketone and uridine diphosphoglucose dehydrogenase by 5-(iodoacetamidoethyl)aminonaphthalene-1-sulfonic acid are fit with just five parameters for each enzyme-modifier pair. In the case of glyceraldehyde-3-phosphate dehydrogenase, the time courses for modification of the yeast enzyme by iodoacetic acid and the rabbit-muscle enzyme by 3,3,3-trifluorobromoacetone are fit with the same model, and parameter values from these fits are used to generate theoretical inactivation curves which are found to agree well with the experimentally measured inactivation. We conclude that half-of-the-sites reactivity, if it is not an artifact of residual heterogeneity, could be a kinetic phenomenon related to metastability of partially ...Continue Reading

References

Dec 16, 1975·Biochemistry·S C MockrinD E Koshland
Oct 15, 1973·Journal of Molecular Biology·W B Stallcup, D E Koshland
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K MoonE L Smith
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·Q H Gibson
Oct 15, 1974·Journal of Molecular Biology·Y OhshimaT Horiuchi
Jan 28, 1971·Journal of Molecular Biology·R A MacQuarrie, S A Bernhard
Oct 28, 1971·Journal of Molecular Biology·J J HopfieldS Ogawa
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y I Henis, A Levitzki
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

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Citations

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D A Schwab, J E Wilson
Nov 7, 2016·The Journal of Biological Chemistry·Alessio BocediGiorgio Ricci

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