Molecular targets for rapid identification of Brucella spp

BMC Microbiology
Vladyslava G RatushnaCynthia J Gibas

Abstract

Brucella is an intracellular pathogen capable of infecting animals and humans. There are six recognized species of Brucella that differ in their host preference. The genomes of the three Brucella species have been recently sequenced. Comparison of the three revealed over 98% sequence similarity at the protein level and enabled computational identification of common and differentiating genes. We validated these computational predictions and examined the expression patterns of the putative unique and differentiating genes, using genomic and reverse transcription PCR. We then screened a set of differentiating genes against classical Brucella biovars and showed the applicability of these regions in the design of diagnostic tests. We have identified and tested set of molecular targets that are associated in unique patterns with each of the sequenced Brucella spp. A comprehensive comparison was made among the published genome sequences of B. abortus, B. melitensis and B. suis. The comparison confirmed published differences between the three Brucella genomes, and identified subsets of features that were predicted to be of interest in a functional comparison of B. melitensis and B. suis to B. abortus. Differentiating sequence regions f...Continue Reading

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Feb 10, 2009·Journal of Bacteriology·Jeffrey T FosterPaul Keim
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Datasets Mentioned

BETA
AF076290
AE017224
AE008918
AE014292

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Artemis
BLAST
BLASTn
MUMmer
ERGO
tblastx
Primer3

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