Abstract
We used cDNA microarray analysis to obtain insights into the mechanisms of action of doxazosin, an alpha(1)-adrenergic receptor antagonist used to treat benign prostatic hyperplasia (BPH). Hierarchical clustering analysis and significance analysis of microarray (SAM) were performed to identify genes differentially expressed between untreated stromal cells cultured from normal tissue and BPH, and changes in gene expression induced by doxazosin. Transcript levels of selected genes were validated by real-time reverse-transcription polymerase chain reaction (RT-PCR). Hierarchical clustering analyses separated untreated normal and BPH cells. Sixty-seven genes whose expression varied at least twofold after doxazosin treatment in both normal and BPH cells were identified, as were 93 genes differentially regulated in normal versus BPH cells. Molecular targets consistent with tumor necrosis factor (TNF)-alpha-related activity were identified. Normal versus BPH stromal cells differ in global gene transcription. Doxazosin induced gene expression changes relevant to proliferation/apoptosis, immune defense, cell-cell signaling/signal transduction, and transcriptional regulation.
References
Nov 14, 1997·British Journal of Urology·R S Kirby, J L Pool
Nov 20, 1997·The Prostate·G YangT C Thompson
Aug 12, 1998·British Journal of Urology·D E Clarke
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jul 14, 1999·Cancer Letters·Y J SurhS J Lee
Oct 12, 2000·The Prostate·D M Peehl, R G Sellers
Jan 11, 2000·Nucleic Acids Research·G SherlockJ M Cherry
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Aug 9, 2001·The Prostate·K Y IlioC Lee
Dec 1, 2001·Free Radical Biology & Medicine·J Nordberg, E S Arnér
Jun 12, 2002·Molecular Biology of the Cell·Michael L WhitfieldDavid Botstein
Feb 11, 2003·The Journal of Urology·Kasapr Keledjian, Natasha Kyprianou
Mar 12, 2003·The Journal of Urology·Natasha Kyprianou
Apr 4, 2003·Oncogene·Anwu ZhouNoelle Sevilir Williams
May 20, 2003·Biochemical Pharmacology·Morag C E McFadyenGraeme I Murray
May 29, 2003·British Journal of Cancer·J V PartinN Kyprianou
Jun 12, 2003·International Journal of Cancer. Journal International Du Cancer·Gregory E WeitsmanRuth Koren
Nov 18, 2003·Molecular Biology of the Cell·Hongjuan ZhaoJames D Brooks
Citations
Sep 4, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Yi-Fan YangMing-Jai Su
Aug 19, 2011·Cell Death & Disease·J JehleD Thomas
May 30, 2007·Prostate Cancer and Prostatic Diseases·T DrewaJ Styczynski
Mar 23, 2013·Journal of Clinical Medicine Research·Georgios PapadopoulosDionisios Mitropoulos
Sep 12, 2009·The American Journal of the Medical Sciences·He-Lin TianYan-Hui Kang
Oct 18, 2006·Journal of Cellular Physiology·Hongjuan ZhaoDonna M Peehl
Oct 17, 2012·Life Sciences·Flávia K DelellaSérgio L Felisbino
Oct 31, 2014·Journal of Molecular Neuroscience : MN·Jian WangChuanlu Jiang
Jan 16, 2008·The Prostate·Chen-Li LaiErik Teske