Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Journal of Fungi
Breno Gonçalves PinheiroAnderson Messias Rodrigues

Abstract

Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 °C and as budding yeasts when cultured at 37 °C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recen...Continue Reading

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Citations

Feb 10, 2021·Journal of Fungi·Alison Felipe Alencar ChavesWagner Luiz Batista
Mar 4, 2021·Journal of Fungi·Breno Gonçalves PinheiroAnderson Messias Rodrigues
Jun 3, 2021·Diagnostics·Victoria PoplinNathan C Bahr

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Methods Mentioned

BETA
scrapings
enzyme-linked immunosorbent assay
biopsy
bronchoalveolar lavage
PCR
PCRs
amplicon
genotyping
biopsies
RAPD

Software Mentioned

single analysis (
MLSA
SNaPshot )

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