PMID: 8584795Nov 1, 1995Paper

Molecular tools for the study of transcriptional regulation in Bacillus anthracis

Research in Microbiology
J C SirardAgnès Fouet

Abstract

Bacillus anthracis produces two toxins composed of three proteins. Genetic tools were constructed to study the regulation of toxin synthesis. They included transcriptional fusions with various reporter genes, in replicative and integrative vectors. The reporter gene xylE, encoding catechol 2,3-dioxygenase, may be valuable for screening of strong promoters, as expression of the gene can be visualized directly and the studies of regulation in B. anthracis. Therefore, transcriptional fusions between a lacZ reporter gene and the toxin genes were constructed. Experiments with a multicopy plasmid in trans suggested that the transcriptional activator(s) of the toxin genes were not titrated. B. anthracis strains, which contain pXO1 carrying multiple copies of fusions, were analysed. Expression of the reporter gene was proportional to the fusion copy number. Indeed, single integration of a suicide plasmid can be distinguished from multiple integration according to the level of resistance to an appropriate antibiotic. Finally, recombination in B. anthracis was found to be very efficient (approximately 10(-2) recombinants per transconjugant cell.

References

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Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M M ZukowskiJ P Lecocq
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Citations

Sep 4, 1999·Journal of Applied Microbiology·A FouetM Mock
Sep 4, 1999·Journal of Applied Microbiology·F BrossierJ C Sirard
Dec 12, 2001·Molecular Microbiology·C Guidi-RontaniM Mock
Dec 26, 2001·Journal of Bacteriology·Elke Saile, Theresa M Koehler
Jun 5, 2007·PLoS Pathogens·Ian J GlomskiPierre L Goossens
Mar 15, 2006·Current Opinion in Microbiology·Agnès Fouet, Michèle Mock
Sep 25, 2010·Research in Microbiology·Agnès Fouet
Apr 2, 2015·PLoS Neglected Tropical Diseases·Christophe BrézillonPierre L Goossens
Nov 4, 2004·Biosensors & Bioelectronics·James PannucciRobert B Cary
Jul 18, 2009·Molecular Aspects of Medicine·Agnès Fouet
Oct 19, 2010·Biologie aujourd'hui·Philippe ThullierDominique Vidal
Jul 27, 1999·Journal of Bacteriology·A R Hoffmaster, T M Koehler
Jan 18, 2002·Infection and Immunity·Fabien BrossierMichèle Mock

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