Molecular-Type Specific Multiplex PCR produces a distinct VNII PCR pattern among Cryptococcus neoformans species complex

Medical Mycology
Massimo CogliatiMuriel Cornet

Abstract

Prevalence of molecular type VNII among Cryptococcus neoformans species complex is probably underestimated since it can be distinguished from VNI only using molecular typing methods such as URA5-RFLP, AFLP, MLST, or whole genome sequencing. Previously, we described a multiplex polymerase chain reaction (PCR) method able to identify VNI, VNIV, and VNIII hybrids, but, at that time, VNII molecular type was not described yet. In this study, 16 VNII global isolates were analyzed by our multiplex PCR method, and results showed that it was able to produce a specific pattern for all the studied VNII isolates, which was different from those of VNI, VNIV, and VNIII.

References

Apr 3, 2001·Microbiology·T BoekhoutW Meyer
Feb 27, 2003·Emerging Infectious Diseases·Wieland MeyerUNKNOWN IberoAmerican Cryptococcal Study Group
Aug 3, 2007·Journal of Clinical Microbiology·Massimo CogliatiMaria Anna Viviani
Jan 5, 2013·Medical Mycology·Massimo CogliatiUNKNOWN Fimua Cryptococcosis Network
Feb 28, 2015·Fungal Genetics and Biology : FG & B·Ferry HagenTeun Boekhout

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