Molecular typing and antifungal susceptibility of Exophiala isolates from patients with cystic fibrosis

Journal of Medical Microbiology
Ann PackeuFrançoise Symoens

Abstract

The black yeast Exophiala dermatitidis is a frequent agent of colonization of the lungs of patients with cystic fibrosis (CF). A total of 71 clinical isolates of Exophiala from 13 patients were identified at the species level by sequencing the internal transcribed spacer (ITS) regions 1 and 2 of the rDNA genes and typed by random amplification of polymorphic DNA (RAPD), using two different primers, BG-2 and ERIC-1. In vitro susceptibility of these isolates to some systemic antifungal drugs was investigated using the CLSI method. Almost all the isolates were identified as E. dermatitidis, but long-term colonization with the closely related species E. phaeomuriformis was observed in one patient. No clustering was found according to the geographical origin of the isolates, the isolation date or the antifungal susceptibility. Variations were seen in the susceptibility of studied isolates to antifungals but most of them exhibited low susceptibility to amphotericin B and although some patients were successively colonized by two distinct genotypes, most of the isolates were distributed in patient-specific clusters. This phenomenon may be due to genomic variations of E. dermatitidis in the lung environment of CF patients. These results...Continue Reading

References

May 1, 1992·European Journal of Pediatrics·G KusenbachH Döhmen
Oct 1, 1986·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·T MatsumotoM R McGinnis
Feb 1, 1994·Archives of Disease in Childhood·E J SimmondsE G Evans
Mar 1, 1994·Journal of Clinical Microbiology·H GirardinJ P Latgé
Apr 25, 2000·The Journal of Hospital Infection·F SymoensN Nolard
Dec 1, 2001·Scandinavian Journal of Infectious Diseases·D DiemertR Rennie
May 31, 2002·Journal of Clinical Microbiology·Alain DefontaineJean-Philippe Bouchara
Dec 11, 2002·Mycoses·N A Yurlova, G S de Hoog
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Kim LangfelderAxel A Brakhage
Aug 19, 2004·Respiration; International Review of Thoracic Diseases·R HorréN Schnitzler
Mar 23, 2007·Journal of Clinical Microbiology·J M BainF C Odds
May 7, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L M E VanheeT Coenye
Jun 23, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kuo-Wei ChenShu-Ying Li
Feb 9, 2010·Medical Mycology·Ricardo AraujoLeonor Gusmão
Aug 24, 2010·International Journal of Medical Microbiology : IJMM·Manuel WoltersMartin Christner
Nov 12, 2010·Medical Mycology·Patrick LebecqueFrançoise Symoens

❮ Previous
Next ❯

Citations

Jan 29, 2013·The Journal of Investigative Dermatology·Maria GrumbtPeter Staib
Jun 4, 2014·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S ChalkiasM T Wong
Mar 15, 2014·The International Journal of Biochemistry & Cell Biology·Sanjay H Chotirmall, Noel G McElvaney
Nov 3, 2017·Mycopathologia·Frédéric GrenouilletJean-Philippe Bouchara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.