Abstract
The province of Manitoba, Canada, with a population of approximately 1.3 million, has been experiencing increased incidence of syphilis cases since 2015. In this study, we examined the detection of Treponema pallidum DNA in 354 clinical samples from 2012 to 2016, and determined molecular types and mutations conferring resistance to azithromycin in the polymerase chain reaction (PCR)-positive samples. T. pallidum DNA detection was done by PCR amplification of tpp47, bmp, and polA genes. Syphilis serology results were reviewed for the PCR-positive cases. Molecular typing of syphilis strains was done by analysis of the T, pallidum arp, tpr, and tp0548 gene targets as well as partial sequencing of the 23S rRNA gene for azithromycin resistance. Of the 354 samples tested, 74 individual cases were PCR positive. A result from the treponemal antibody chemiluminescent microparticle immunoassay test was positive in 72 of these cases and that from the Venereal Disease Research Laboratory testing was positive in 66. Mutations conferring resistance to azithromycin were found in all 74 PCR-positive samples. Molecular typing was completed on 57 PCR-positive samples, and 12 molecular types were identified with 14d/g found in 63.2%. Increased st...Continue Reading
References
Oct 17, 1998·Sexually Transmitted Diseases·A PillayS A Morse
Jul 13, 2004·The New England Journal of Medicine·Sheila A LukehartJeffrey D Klausner
Feb 1, 2006·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Muhammad G Morshed, Hugh D Jones
Jan 15, 2008·Current Opinion in Infectious Diseases·Kenneth A Katz, Jeffrey D Klausner
May 12, 2009·Journal of Medical Microbiology·Petra MatejkováDavid Smajs
Jun 12, 2010·Sexually Transmitted Diseases·Irene E MartinAmeeta E Singh
Sep 28, 2010·The Journal of Infectious Diseases·Christina M MarraSheila A Lukehart
Jan 21, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B-R WuS-Y Chang
Dec 9, 2014·Current Opinion in Infectious Diseases·Craig Tipple, Graham P Taylor
Mar 24, 2015·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Raymond Sw TsangKamran Kadkhoda
Mar 24, 2015·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Paul N LevettMuhammad Morshed
Jul 17, 2015·Journal of Clinical Microbiology·John R M SmithKamran Kadkhoda
Dec 4, 2015·MMWR. Recommendations and Reports : Morbidity and Mortality Weekly Report. Recommendations and Reports·William A BowerUNKNOWN Centers for Disease Control and Prevention (CDC)
Apr 23, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Rafil KhairullinMagnus Unemo
May 20, 2016·Journal of Clinical Microbiology·Phillip ReadBasil Donovan
Jun 21, 2016·Sexually Transmitted Diseases·Juan Antonio FloresCarlos Fernando Caceres
Feb 25, 2017·PloS One·Lucía Gallo VauletDavid Šmajs
Mar 9, 2017·IDCases·Gabriel AnidKamran Kadkhoda
Mar 31, 2017·Microbial Cell·Lola V Stamm
Apr 7, 2017·MMWR. Morbidity and Mortality Weekly Report·Alex de VouxKyle T Bernstein
Feb 4, 2016·Canada Communicable Disease Report = Relevé Des Maladies Transmissibles Au Canada·C RossP Plourde