PMID: 20625238Jul 14, 2010Paper

Molecular typing of avian pathogenic Escherichia coli O78 strains in Japan by using multilocus sequence typing and pulsed-field gel electrophoresis

The Journal of Veterinary Medical Science
Manao OzawaTetsuo Asai

Abstract

Avian pathogenic Escherichia coli (APEC) serogroup O78 isolates in Japan were characterized using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Thirty-four of the serotype O78 isolates were clustered into 11 PFGE types with 80% similarity and were classified into 5 sequence types (STs), ST23, ST117, ST155, ST369 and 1 novel ST (ST1645). The most dominant ST was ST23 (54.3%). Fluoroquinolone-resistant strains belonged to ST23 (9 strains), ST155 (3 strains) and ST117 (1 strain). The combined findings of MLST and PFGE suggested that 1 genotype belonging to ST23 was the genotype specific to fluoroquinolone-resistant strains. These results indicate that the combination of MLST and PFGE is useful for conducting epidemiological studies on APEC O78 strains.

Citations

Oct 7, 2014·Diagnostic Microbiology and Infectious Disease·Joseane Cristina FerreiraAna Lúcia Costa Darini
Oct 30, 2012·Veterinary Microbiology·Hyuk-Joon KwonJae-Hong Kim
Sep 1, 2015·PloS One·Nathan L BachmannAdam Polkinghorne
Mar 7, 2019·Frontiers in Microbiology·Christian SchweizerYvonne Pfeifer

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