Molecular typing of Pasteurella pneumotropica isolated from rodents by amplified 16S ribosomal DNA restriction analysis and pulsed-field gel electrophoresis

Microbiology and Immunology
Hiraku SasakiTakuo Sawada

Abstract

A total of 52 isolates of Pasteurella pneumotropica obtained from rodents were examined for their genetic heterogeneity. On the basis of DNA restriction analysis, including amplified 16S ribosomal DNA restriction analysis (ARDRA) and pulsed-field gel electrophoresis (PFGE), differences were identified among the isolates. ARDRA typing with Hae III revealed 4 different banding patterns of the P. pneumotropica isolates. Eighty-two percent of the 23 isolates identified as a-1 were derived from mice, whereas all the isolates identified as a-3 were derived from rats. Most of the isolates, which showed hemolytic activity on blood agar, obtained from mice and rats, were identified as a-2 and a-4, respectively. By restriction analysis of genomic DNA, Apa I and Not I digestion differentiated 9 variants and an undiscriminating group. However, no close relation with regard to the phenotypic characteristics was observed among the variants. The isolates identified as a-2 and a-4 could not be distinguished by PFGE analysis. DNA restriction analysis revealed that the genetic diversity of the P. pneumotropica isolates was more complex than the phenotypic characteristics among the species, and that at least the P. pneumotropica isolates were cle...Continue Reading

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Citations

Jan 15, 2008·Antonie van Leeuwenhoek·Matteo BusconiCorrado Fogher
Feb 24, 2009·Antonie van Leeuwenhoek·Hiraku SasakiKen-ichi Yagami
Nov 19, 2010·BMC Veterinary Research·Yasuko HirakawaHiromi Amao
Sep 7, 2012·Aquatic Biosystems·Polpass Arul Jose, Solomon Robinson David Jebakumar
Jan 20, 2012·Comparative Immunology, Microbiology and Infectious Diseases·André Bleich, Axel Kornerup Hansen
Sep 8, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hiraku SasakiKen-Ichi Yagami
May 8, 2008·Avian Diseases·Alfredo Mendoza-EspinozaAmparo I Zavaleta

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