Molecular typing of Staphylococcus aureus isolated from blood on the basis of coagulase gene polymorphism and toxin genes

The Korean journal of laboratory medicine
Yong Kyun KimKyu Man Lee

Abstract

Coagulase is produced by all strains of Staphylococcus aureus. The 3' coding region of the coagulase (coa) gene contains varying numbers of 81 bp tandem repeats. S. aureus produces a variety of extracellular protein toxins. Here, we typed S. aureus strains isolated from blood by coa gene restriction fragment length polymorphism (RFLP) patterns and toxin gene profiles. A total of 120 strains of S. aureus were isolated from blood cultures during 2003-2006 at Kangdong Sacred Heart Hospital. The isolates were typed by PCR RFLP analysis of the coa gene and by multiplex PCR for detection of genes encoding enterotoxins (sea, seb, sec, sed, and see), toxic shock syndrome toxin-1 (tst), exfoliative toxins (eta and etb), mecA and femA. All the S. aureus strains were classified into 16 types on the basis of coa gene RFLP and could be further differentiated into 34 types according to the combined patterns of coa gene RFLP and toxin gene profiles. Of 85 methicillin-resistant S. aureus (MRSA) strains, 43 (50.6%) and 36 (42.4%) belonged to the RFLP pattern L5 and pattern L1, respectively. MRSA strains belonging to pattern L5 frequently carried tst (93.0%) or sec gene (81.4%), and strains belonging to pattern L1 frequently carried sea (88.9%) ...Continue Reading

References

Jul 1, 1992·Journal of Clinical Microbiology·S H GohA W Chow
Aug 1, 1992·Journal of Clinical Microbiology·M J Carles-NuritM Ramuz
Jan 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N LehnM Krönke
Feb 1, 1994·Journal of Clinical Microbiology·F C TenoverR Hollis
Apr 1, 1996·The Journal of Antimicrobial Chemotherapy·C LawrenceR Leclercq
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Jan 11, 2000·Clinical Microbiology Reviews·M M DingesP M Schlievert
Sep 25, 2002·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Junichiro NishiIkuro Maruyama
Mar 21, 2007·Chemical Immunology and Allergy·Damien ThomasGerard Lina

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