Molecular variability of the adhesin-encoding gene pvpA among Mycoplasma gallisepticum strains and its application in diagnosis

Journal of Clinical Microbiology
T LiuS H Kleven

Abstract

Mycoplasma gallisepticum is an important pathogen of chickens and turkeys that causes considerable economic losses to the poultry industry worldwide. The reemergence of M. gallisepticum outbreaks among poultry, the increased use of live M. gallisepticum vaccines, and the detection of M. gallisepticum in game and free-flying song birds has strengthened the need for molecular diagnostic and strain differentiation tests. Molecular techniques, including restriction fragment length polymorphism of genomic DNA (RFLP) and PCR-based random amplification of polymorphic DNA (RAPD), have already been utilized as powerful tools to detect intraspecies variation. However, certain intrinsic drawbacks constrain the application of these methods. The main goal of this study was to determine the feasibility of using an M. gallisepticum-specific gene encoding a phase-variable putative adhesin protein (PvpA) as the target for molecular typing. This was accomplished using a pvpA PCR-RFLP assay. Size variations among PCR products and nucleotide divergence of the C-terminus-encoding region of the pvpA gene were the basis for strain differentiation. This method can be used for rapid differentiation of vaccine strains from field isolates by amplificatio...Continue Reading

References

Jul 1, 1996·Infection and Immunity·S F DalloJ B Baseman
Dec 1, 1996·Revue Scientifique Et Technique·K G Whithear
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S RazinY Naot
Mar 31, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·B R CharltonR Yamamoto

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Citations

Feb 21, 2003·Avian Pathology : Journal of the W.V.P.A·Dusan Bencina
Apr 19, 2011·Avian Pathology : Journal of the W.V.P.A·Alexander V SpryginVladimir V Drygin
Aug 5, 2011·Avian Pathology : Journal of the W.V.P.A·Saad Gharaibeh, Alaa Hailat
Feb 4, 2010·Infection and Immunity·S M SzczepanekS J Geary
Nov 9, 2004·Avian Diseases·S H KlevenE Wallner-Pendleton
May 2, 2012·Avian Diseases·N M Ferguson-NoelS H Kleven
Mar 9, 2011·Veterinary Microbiology·Saad Gharaibeh, Mohammad Al-Rashdan
Nov 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ozlem BüyüktanirNevzat Yurdusev
Feb 6, 2007·Avian Diseases·Cynthia M Boettger, John E Dohms
Jul 7, 2018·Journal of Veterinary Research·Syed Ehtisham-Ul-HaqueMuhammad Younus
Oct 13, 2018·Pakistan Journal of Biological Sciences : PJBS·Gamal A M YounisAmira F Glal
Apr 13, 2021·Animal Biotechnology·Jay Prakash YadavSandip Kumar Khurana

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