Molecular variation between the alpha-toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animals.

Microbiology
A GinterRichard W Titball

Abstract

The alpha-toxin produced by the type strain of Clostridium perfringens (NCTC 8237) was shown to differ from the alpha-toxins produced by most strains of C. perfringens isolated from man and from calves with respect to reactivity with a neutralizing monoclonal antibody (DY2F5D11). The difference in antibody binding correlated with three differences in the deduced amino acid sequence (Ala174 to Asp174; Thr177 to Ala177; Ser335 to Pro335) of the alpha-toxins. Using octapeptides synthesized on the basis of the amino acid sequences from these regions of variability, it was shown that the Ala174 to Asp174 change had the greatest effect on reducing the binding of monoclonal antibody DY2F5D11 to the alpha-toxin. These differences did not affect the enzymic or toxic properties of the protein. However, the phospholipase C activity of the alpha-toxin produced by strain NCTC 8237 was more susceptible to inactivation by chymotrypsin. The changes in amino acid sequence did not affect the ability of a C-terminal domain vaccine, derived from the alpha-toxin of strain NCTC 8237, to induce protection against the alpha-toxin from a bovine enteric strain of C. perfringens.

References

Aug 1, 1975·Canadian Journal of Microbiology·J R Lapointe, V Fredette
Dec 1, 1991·Infection and Immunity·A J LoganD C Kelly
Apr 14, 1989·Biochemical and Biophysical Research Communications·A OkabeH Hayashi
Aug 1, 1989·Antonie van Leeuwenhoek·M Van Damme-JongstenS Notermans
Jul 1, 1986·British Journal of Pharmacology·Y FujiiJ Sakurai
Dec 1, 1965·Biochemistry·J R Clark, L Cunningham
Jun 1, 1993·Microbiological Reviews·R W Titball

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Citations

Dec 19, 2003·Avian Pathology : Journal of the W.V.P.A·Atle LovlandAtle Lillehaug
Jan 19, 1999·Annual Review of Microbiology·J I Rood
Feb 27, 2004·International Journal of Food Microbiology·Sufian F Al-KhaldiVladimir Chizhikov
Oct 20, 2009·Vaccine·Richard W Titball
Sep 29, 2007·Journal of Ethnopharmacology·K VijayanarayanaE V S Subrahmanyam
Aug 5, 2006·Anaerobe·R W TitballA K Basak
Sep 10, 2005·Microbiology·Richard W Titball
Apr 22, 1999·Trends in Microbiology·L PetitM R Popoff
Mar 18, 1999·Toxicon : Official Journal of the International Society on Toxinology·M L Hale, B G Stiles
Apr 1, 1997·Trends in Microbiology·J G Songer
Mar 9, 2004·Journal of Clinical Microbiology·Scott A SheedyRobert J Moore

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