PMID: 9660067Jul 11, 1998Paper

Molecular variation in the nucleoprotein gene (ORF7) of the porcine reproductive and respiratory syndrome virus (PRRSV)

Virus Research
A Le GallA Jestin

Abstract

The nucleoprotein gene (ORF7) of 15 European isolates of porcine reproductive and respiratory syndrome virus (PRRSV) was sequenced and compared with corresponding sequences of other PRRSV isolates (2 European and 13 American) and one isolate each of other arteriviruses (the lactate dehydrogenase elevating virus (LDV), the simian haemorrhagic fever virus (SHFV) and the equine arteritis virus (EAV)). Their phylogenetic relationships were established using neighbour-joining and parsimony methods. Four lineages (PRRSV, LDV, SHFV and EAV) were discriminated. Two genotypes of PRRSV, European and American, could be further identified. The European genotype of PRRSV was highly conserved. Analysis of the nucleotide and amino acid substitutions in PRRSV ORF7 revealed four stable regions, probably conserved because of their requirement for nucleocapsid function and/or structure. No constant mutations accumulation in the ORF7 could be determined precisely when either synonymous or non-synonymous mutations were studied. Passage of the European PRRSV in vivo had little influence on the ORF7 sequence: only a small number of synonymous substitutions in ORF7 was detectable, confirming its low variability.

References

Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D A BenfieldD Chladek
Jul 1, 1991·The Veterinary Quarterly·G WensvoortF Wagenaar
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May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Apr 1, 2000·Avian Pathology : Journal of the W.V.P.A·D S GohmM A Hofmann
Oct 9, 2002·Journal of Virology·Roeland WieringaPeter J M Rottier
Sep 15, 2010·Virus Research·Mang ShiFrederick Chi-Ching Leung
Jul 19, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kerstin WernikeBernd Hoffmann
Dec 23, 2017·Asian-Australasian Journal of Animal Sciences·Jong-Nam OhChang-Kyu Lee

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