Molecular verification of transplacental transmission of Theileria lestoquardi in goat

Parasitology Research
Bijan EsmaeilnejadNasser Hajipour

Abstract

Ovine and caprine malignant theileriosis (OCMT), a critical condition in small ruminant production, causes lethal infections. In September 2016, a total number of 400 goats of Marghoz breed (the largest population of goat breed in Iran), in northwest of Iran, were examined for approximately 25 pre-partum abortions and 7 goats' mortality for a period of 3 days. A dead goat and her aborted fetus were brought into the Urmia Veterinary Hospital for further diagnostic investigations. The microbiological assessments including direct microscopical examination of the vaginal discharges and placentome with respect to the differential staining (Gram's staining method), conventional pure culturing, fetal abomasal contents, and the liver were negative. Microscopic examinations of blood smears of the goat and the fetus revealed characteristic of Theileria spp. piroplasms and impression smear samples from goat liver and fetal spleen were positive for Theileria Koch blue bodies. DNA analysis was performed using PCR technique and specific primers derived from the nucleotide sequences of 18S rRNA gene of T. lestoquardi; following extraction from blood samples, placentome, goat liver, and spleen of the fetus. The amplified DNA was sequenced afte...Continue Reading

References

May 13, 2014·Veterinary Parasitology·Amir ZakianFardokht Rashidi
May 3, 2015·Tropical Animal Health and Production·Vikrant SudanDaya Shanker
Jun 13, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Vikrant SudanDaya Shanker

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Citations

Dec 4, 2020·Animals : an Open Access Journal From MDPI·Snorre Stuen
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Ahmed Magdy SelimAl Shymaa Al Sayed Shwareb

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Datasets Mentioned

BETA
MG208059
KY352037.1
KC778786.1
JQ917458.1

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST

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