Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro

International Journal of Biological Macromolecules
Xiangtao MengPengcheng Li

Abstract

Aminoethyl modified chitosan derivatives (AEMCSs) with different molecular weight (Mw) were synthesized by grafting aminoethyl group on different molecular weight chitosans and chitooligosaccharide. FTIR, (1)H NMR, (13)C NMR, elemental analysis and potentiometric titration results showed that branched polyethylimine chitosan was synthesized. Clinical Laboratory Standard Institute (CLSI) protocols were used to determine MIC for Gram-negative strain of Escherichia coli under different pH. The antibacterial activity of the derivatives was significantly improved compared with original chitosans, with MIC values against E. coli varying from 4 to 64 μg/mL depending on different Mw and pH. High molecular weight seems to be in favor of stronger antibacterial activity. At pH 7.4, derivatives with Mw above 27 kDa exhibited equivalent antibacterial activity (16 μg/mL), while oligosaccharide chitosan derivative with lower Mw (~1.4 kDa) showed decreased MIC of 64 μg/mL. The effect of pH on antibacterial activity is more complicated. An optimal pH for HAEMCS was found around 6.5 to give MIC as low as 4 μg/mL, while higher or lower pH compromised the activity. Cell integrity assay and SEM images showed evident cell disruption, indicating memb...Continue Reading

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Citations

Jul 12, 2013·BioMed Research International·Antonio PeñaMartha Calahorra
Aug 11, 2016·Bioorganic & Medicinal Chemistry Letters·Linfeng HuPengcheng Li
Feb 13, 2013·Carbohydrate Polymers·Zhongshan ZhangHuimin Qi
Jul 30, 2016·International Journal of Biological Macromolecules·Bing-Jie ChenTao Zhou
Jun 11, 2019·Drug and Chemical Toxicology·Xiaofei HePengcheng Li
Apr 12, 2019·International Journal of Molecular Sciences·Priyanka SahariahMár Másson
Jun 14, 2019·Natural Product Research·Ruge CaoZhenyu Zhao
Nov 28, 2019·Medical Mycology·Ana I LopesManuela E Pintado
Apr 6, 2021·Frontiers in Cellular and Infection Microbiology·Homa BoroumandHamed Mirzaei
Jul 25, 2021·International Journal of Molecular Sciences·Cristina ArdeanVirgil Musta
Sep 22, 2017·Biomacromolecules·Priyanka Sahariah, Már Másson

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