Molecular weight characterization of single globular proteins using optical nanotweezers

The Analyst
Skyler Wheaton, Reuven Gordon

Abstract

We trap a set of molecular weight standard globular proteins using a double nanohole optical trap. The root mean squared variation of the trapping laser transmission intensity gives a linear dependence with the molecular weight, showing the potential for analysis of globular proteins. The characteristic time of the autocorrelation of the trapping laser intensity variations scales with a -2/3 power dependence with the volume of the particle. A hydrodynamic laser tweezer model is used to explain these dependencies. Since this is a single particle technique that operates in solution and can be used to isolate an individual particle, we believe that it provides an interesting alternative to existing analysis methods and shows promise to expand the capabilities of protein related studies to the single particle level.

References

Jan 1, 1968·Advances in Protein Chemistry·C Tanford
May 1, 1969·Biochimica Et Biophysica Acta·A BezkorovainyD Grohlich
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jul 2, 1999·Biophysical Journal·H MorganN G Green
Jan 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Debora BonenfantPaul Jenoe
Dec 17, 2011·Nano Letters·Yuanjie Pang, Reuven Gordon
May 25, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Kai Wang, Kenneth B Crozier
Jun 22, 2013·Optics Express·Pavel N MelentievVictor I Balykin
Sep 21, 2013·Biomedical Optics Express·Ahmed A Al BalushiReuven Gordon
Jun 19, 2014·Biomedical Optics Express·Abhay Kotnala, Reuven Gordon
Sep 12, 2014·Nano Letters·Ahmed A Al Balushi, Reuven Gordon
Nov 20, 2014·Nanotechnology·Ana Zehtabi-OskuieReuven Gordon
Dec 10, 2014·Optics Letters·Ryan M GelfandReuven Gordon

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Citations

Sep 23, 2016·Nano Letters·A A P TrichetJ M Smith
Aug 4, 2018·Nanotechnology·Muhammad Usman RazaGeorge Alexandrakis
Jun 6, 2019·Optics Express·Adarsh Lalitha RavindranathReuven Gordon
Mar 19, 2021·Light, Science & Applications·Yuquan ZhangXiaocong Yuan
Apr 3, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Joshua D KolbowSang-Hyun Oh
May 10, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Souvik Ghosh, Ambarish Ghosh

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