Molecular weight distribution evaluation of polysaccharides and glycoconjugates using analytical ultracentrifugation.

Macromolecular Bioscience
Stephen E HardingGordon A Morris

Abstract

We review here the advances that have been made in methodology using the analytical ultracentrifuge for characterising polydisperse polymer systems with a quasi-continuous distribution of molecular weight. These advances include improved ways of defining the weight and z-average molecular weights of a distribution from sedimentation equilibrium experiments and hence the ratio Mz/Mw, off-line calibration of size exclusion chromatography by sedimentation equilibrium and conversion of a sedimentation coefficient distribution into a molecular weight distribution. Although these methods are applicable to other polymeric systems, we focus on polysaccharides, mucins and other glycoconjugates, systems which particularly benefit from the availability of recently available long optical path length cells for the minimisation of complications through thermodynamic non-ideality.

References

Jan 1, 1989·Advances in Carbohydrate Chemistry and Biochemistry·S E Harding
Oct 1, 1986·The Biochemical Journal·J K SheehanI Carlstedt
Jan 1, 1967·Progress in Biophysics and Molecular Biology·J M Creeth, R H Pain
Dec 1, 1982·Journal of Biochemical and Biophysical Methods·J M Creeth, S E Harding
Oct 1, 1980·Biochemical Society Transactions·J M Creeth
Mar 1, 1964·Biochemistry·D A YPHANTIS

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Citations

Jun 9, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Josua Timotheus OberlerchnerAntje Potthast
Sep 3, 2010·Journal of Drug Targeting·Stephen E Harding
Mar 19, 2013·Carbohydrate Polymers·Ali PourjavadiSeyed Hassan Hosseini

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