Molecularly imprinted coated graphene oxide solid-phase extraction monolithic capillary column for selective extraction and sensitive determination of phloxine B in coffee bean

Analytica Chimica Acta
Haiyun ZhaiLu Huang

Abstract

A method was developed to sensitively determine phloxine B in coffee bean by molecularly imprinted polymers (MIPs) coated graphene oxide (GO) solid-phase extraction (GO-MISPE) coupled with high-performance liquid chromatography and laser-induced fluorescence detection (HPLC-LIF). The GO-MISPE capillary monolithic column was prepared by water-bath in situ polymerization, using GO as supporting material, phloxine B, methacrylic acid (MAA), and ethylene dimethacrylate (EDMA) as template, functional monomer, and cross-linker, respectively. The properties of the homemade GO-MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions. The GO-MIPs were characterized by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FT-IR). The mean recoveries of phloxine B in coffee bean ranged from 89.5% to 91.4% and the intra-day and inter-day relative standard deviation (RSD) values all ranged from 3.6% to 4.7%. Good linearity was obtained over 0.001-2.0 μg mL(-1) (r=0.9995) with the detection limit (S/N=3) of 0.075 ng mL(-1). Under the selected conditions, enrichment factors of over 90-fold were obtained and extraction on the monolithic column effectively clea...Continue Reading

References

May 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Karel StulíkPavel Coufal
Dec 12, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mehran JavanbakhtBehrouz Akbari-adergani

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Citations

Dec 3, 2015·Journal of Chromatography. a·Fang LiZhao-Sheng Liu
Oct 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beatriz Fresco-CalaSoledad Cárdenas
Apr 28, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Haiyun ZhaiQing Zhou
May 6, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hailong YuJing Wang

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