Molecularly imprinted layer-coated monodisperse spherical silica microparticles toward affinity-enrichment of isoflavonoid glycosides from Radix puerariae

The Analyst
Jing LuoZhongping Zhang

Abstract

This paper reports the preparation of puerarin (PR) imprinted layer-coated silica microparticles toward selective recognition of PR and fast affinity-enrichment of the main isoflavonoid glycosides from the crude extract of Radix puerariae. Before the preparation, quantum mechanics (QM) method was applied to identify three kinds of common functional monomers capable of interaction with PR and then predicted optimal functional monomer (acrylamide, AA) and the relative molar ratio of template to functional monomer (PR/AA, 1:4). The obtained PR-imprinted silica microparticles were evaluated by transmission electron microscope (TEM) and rebinding experiments, exhibiting good morphology and high binding affinity to PR. Meanwhile, the rebinding amount of the imprinted microparticles to PR was nearly 2.1-folds that of non-imprinted microparticles. When the PR-imprinted microspheres were used as packing materials for solid-phase extraction, the recovery yields of PR, daidzin (DD) and genistin (GS) were simultaneously up to 90% by one-step extraction from the crude extract of Radix puerariae. Additionally, the PR-imprinted microparticles could be re-used for at least 5 times without losing any extraction efficiency. These results indicat...Continue Reading

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Citations

Jun 20, 2014·Journal of Pharmaceutical and Biomedical Analysis·Zhaozhou LiJunjie Sun
Jan 2, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hai-Dong GuoYu-Xian Guo
Aug 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ya-Jun YangJian-Yong Li

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