Molecularly imprinted polymer beads for clean-up and preconcentration of β-lactamase-resistant penicillins in milk

Analytical and Bioanalytical Chemistry
Javier L UrracaMaria C Moreno-Bondi

Abstract

This work describes the development and application of class-selective molecularly imprinted polymers (MIPs) for the analysis of beta-lactamase-resistant penicillins, namely cloxacillin (CLOXA), oxacillin (OXA), and dicloxacillin (DICLOXA), in milk samples. Our method is based on molecularly imprinted solid-phase extraction (MISPE) coupled to high-performance liquid chromatography (HPLC) with diode-array detection (DAD). 2-Biphenylylpenicillin (2BPEN), a surrogate with a close resemblance to beta-lactamase-resistant penicillins in terms of size, shape, hydrophobicity, and functionality, was synthesized and used as the template for the polymer synthesis. A MIP library was prepared and screened to select the optimum functional monomer, N-(2-aminoethyl)methacrylamide, and cross-linker, trimethylolpropane trimethacrylate, that provided the best recognition for the target antibiotics. For the MISPE application, the MIPs were prepared in the form of microspheres, using porous silica beads (40-75 μm) as sacrificial scaffolds. The developed MISPE method enables efficient extraction from aqueous samples and analysis of the antimicrobials, when followed by a selective washing with 2 mL acetonitrile-water (20:80 v/v) and elution with 1 mL...Continue Reading

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Citations

Feb 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dimitrios Bitas, Victoria Samanidou
Aug 4, 2020·Critical Reviews in Food Science and Nutrition·Seyed Amin KhatibiMohammad Reza Siahi-Shadbad
Mar 23, 2017·Analytical and Bioanalytical Chemistry·Michał CegłowskiGrzegorz Schroeder

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