Molecularly imprinted polymer for caffeic acid by precipitation polymerization and its application to extraction of caffeic acid and chlorogenic acid from Eucommia ulmodies leaves

Journal of Pharmaceutical and Biomedical Analysis
Chitose MiuraJun Haginaka

Abstract

Molecularly imprinted polymers (MIPs) for caffeic acid (CA) were prepared using 4-vinylpyridine and methacrylamide (MAM) as functional monomers, divinylbenzene as a crosslinker and acetonitrile-toluene (3:1, v/v) as a porogen by precipitation polymerization. The use of MAM as the co-monomer resulted in the formation of microsphere MIPs and non-imprinted polymers (NIPs) with ca. 3- and 5-μm particle diameters, respectively. Binding experiments and Scatchard analyses revealed that the binding capacity and affinity of the MIP to CA are higher than those of the NIP. The retention and molecular-recognition properties of the prepared MIPs were evaluated using water-acetonitrile and sodium phosphate buffer-acetonitrile as mobile phases in hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography, respectively. In HILIC mode, the MIP showed higher molecular-recognition ability for CA than in reversed-phase mode. In addition to shape recognition, hydrophilic interactions seem to work for the recognition of CA on the MIP in HILIC mode, while hydrogen bonding and hydrophobic interactions seem to work for the recognition of CA in reversed-phase mode. The MIP had a specific molecular-recognition ability for CA in HILI...Continue Reading

References

Jun 24, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·T Takeuchi, J Haginaka
Oct 1, 2010·Trends in Biotechnology·Alessandro PomaSergey A Piletsky
Jun 19, 2013·Critical Reviews in Food Science and Nutrition·Rohit Upadhyay, L Jagan Mohan Rao
Aug 16, 2013·Analytical Chemistry·Romana Schirhagl
Jan 10, 2014·Talanta·Aytaç GültekinRıdvan Say
Dec 4, 2014·Analytica Chimica Acta·Mahavir Prasad Tiwari, Amrita Prasad

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Citations

Oct 2, 2020·BioTechniques·Zahra El-SchichAnette Gjörloff Wingren
Mar 28, 2019·Combinatorial Chemistry & High Throughput Screening·Jane Ru ChoiAlistair C Cowie
Apr 28, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Guotong QinFangqing Yue
Jul 6, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zilong ChenXin Yang

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