Molecularly imprinted polymers applied to the clean-up of zearalenone and alpha-zearalenol from cereal and swine feed sample extracts

Analytical and Bioanalytical Chemistry
J L UrracaMaría C Moreno-Bondi

Abstract

A molecularly imprinted polymer prepared using 1-allylpiperazine (1-ALPP) as the functional monomer, trimethyltrimethacrylate (TRIM) as the crosslinker and the zearalenone (ZON)-mimicking template cyclododecanyl-2,4-dihydroxybenzoate (CDHB) has been applied to the clean-up and preconcentration of this mycotoxin (zearalenone) and a related metabolite, alpha-zearalenol (alpha-ZOL), from cereal and swine feed sample extracts. The extraction of ZON and alpha-ZOL from the food samples was accomplished using pressurized liquid extraction (PLE) with MeOH/ACN (50:50, v/v) as the extraction solvent, at 50 degrees C and 1500 psi. The extracted samples were cleaned up and preconcentrated through the MIP cartridge and analyzed using HPLC with fluorescence detection (lambda (exc)=271/ lambda (em)=452 nm). The stationary phase was a polar endcapped C18 column, and ACN/MeOH/water 10/55/35 (v/v/v, 15 mM ammonium acetate) at a flow rate of 1.0 mL min(-1) was used as the mobile phase. The method was applied to the analysis of ZON and alpha-ZOL in wheat, corn, barley, rye, rice and swine feed samples fortified with 50, 100 and 400 ng g(-1) of both mycotoxins, and it gave recoveries of between 85 and 97% (RSD 2.1-6.7%, n=3) and 87-97% (RSD 2.3-5.6...Continue Reading

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Citations

Mar 21, 2009·Analytical and Bioanalytical Chemistry·Chris M Maragos
Jun 2, 2009·Analytical and Bioanalytical Chemistry·Gordon S Shephard
Jul 14, 2011·Analytical and Bioanalytical Chemistry·Renata Gadzała-KopciuchBogusław Buszewski
Feb 21, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Rudolf KrskaColin Crews
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·D De SmetS De Saeger
Sep 15, 2014·Journal of Chromatography. a·Michał SzumskiBogusław Buszewski
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Apr 6, 2006·Journal of Chromatography. a·J L UrracaM C Moreno-Bondi
Apr 4, 2015·Critical Reviews in Food Science and Nutrition·Lijuan XieYibin Ying
Jan 7, 2015·Critical Reviews in Analytical Chemistry·Katarzyna KwaśniewskaKrzysztof Cendrowski
May 21, 2008·Biosensors & Bioelectronics·Javier L UrracaMaría C Moreno-Bondi
Jul 1, 2010·Prikladnaia biokhimiia i mikrobiologiia·A E UrusovB B Dzantiev
May 3, 2016·Materials Science & Engineering. R, Reports : a Review Journal·Michael C KoettingNicholas A Peppas

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