Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

ACS Applied Materials & Interfaces
Zijie Zhang, Juewen Liu

Abstract

Molecularly imprinted polymers (MIPs) are produced in the presence of a template molecule. After removing the template, the cavity can selectively rebind the template. MIPs are attractive functional materials with a low cost and high stability, but traditional MIPs often suffer from low binding affinity. This study employs DNA aptamer fragments as macromonomers to improve MIPs. The DNA aptamer for adenosine was first split into two halves, fluorescently labeled, and copolymerized into MIPs. With a fluorescence quenching assay, the importance of imprinting was confirmed. Further studies were carried out using isothermal titration calorimetry (ITC). Compared to the mixture of the free aptamer fragments, their MIPs doubled the binding affinity. Each free aptamer fragment alone cannot bind adenosine, whereas MIPs containing each fragment are effective binders. We further shortened one of the aptamer fragments, and the DNA length was pushed to as short as six nucleotides, yielding MIPs with a dissociation constant of 27 μM adenosine. This study provides a new method for preparing functional MIP materials by combining high-affinity biopolymer fragments with low-cost synthetic monomers, allowing higher binding affinity and providing a...Continue Reading

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Citations

Oct 14, 2016·Sensors·Oliver Hayden
Feb 15, 2019·Small·Zijie Zhang, Juewen Liu
Oct 12, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Woo Seok JinnSeungjoo Haam
Aug 10, 2017·Organic & Biomolecular Chemistry·Wenhu ZhouJuewen Liu
Jan 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiao-Yu SunYan-Ping Shi
Apr 29, 2020·Bulletin of Environmental Contamination and Toxicology·Ji LeiZongxiao Li
Sep 4, 2020·Advanced Materials·Jie Deng, Andreas Walther
Apr 13, 2021·Critical Reviews in Analytical Chemistry·Kang Wei-QiFeng Xue-Song
Jun 20, 2021·Advanced Materials·Maryam ArabiLingxin Chen
Feb 9, 2017·Accounts of Chemical Research·Heidi R CulverNicholas A Peppas
Sep 29, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Yuqing LiJuewen Liu
Apr 13, 2018·Analytical Chemistry·Qiusha LiZheng-Zhi Yin
Nov 24, 2020·ACS Sensors·Jakub KaleckiPiyush S Sharma

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