Molecules and methods for super-resolution imaging.

Methods in Enzymology
Michael A ThompsonW E Moerner

Abstract

By looking at a fluorescently labeled structure one molecule at a time, it is possible to side-step the optical diffraction limit and obtain "super-resolution" images of small nanostructures. In the Moerner Lab, we seek to develop both molecules and methods to extend super-resolution fluorescence imaging. Methodologies and protocols for designing and characterizing fluorophores with switchable fluorescence required for super-resolution imaging are reported. These fluorophores include azido-DCDHF molecules, covalently linked Cy3-Cy5 dimers, and also the first example of a photoswitchable fluorescent protein, enhanced yellow fluorescent protein (EYFP). The imaging of protein superstructures in living Caulobacter crescentus bacteria is used as an example of the power of super-resolution imaging by single-molecule photoswitching to extract information beyond the diffraction limit. Finally, a new method is described for obtaining three-dimensional super-resolution information using a double-helix point-spread function.

Citations

Oct 13, 2010·Journal of the American Chemical Society·Hsiao-lu D LeeW E Moerner
Jul 4, 2012·Journal of Pathology Informatics·Yu ChenLiron Pantanowitz
May 15, 2012·Annual Review of Biophysics·Michael A ThompsonW E Moerner
Jul 7, 2011·International Journal of Molecular Sciences·Sheng-Wen Chiu, Mark C Leake
Dec 1, 2015·Faraday Discussions·W E MoernerQuan Wang
Nov 14, 2014·Genes·Steven J SchnellWeidong Yang
Sep 11, 2012·Nature Methods·Anna B LovelandAntoine M van Oijen
Mar 15, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sven van TeeffelenZemer Gitai
May 31, 2012·Chemistry : a European Journal·Janet CusidoFrançisco M Raymo
Aug 9, 2013·Physical Chemistry Chemical Physics : PCCP·Alexandre Fürstenberg, Mike Heilemann
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Oct 31, 2012·Chemistry : a European Journal·Erhan DenizSalvatore Sortino
Mar 9, 2021·The Journal of Physical Chemistry. B·Jennifer M UrbanTodd D Krauss
Dec 25, 2010·Journal of the American Chemical Society·Ibrahim YildizFrançisco M Raymo
May 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Erhan DenizFrançisco M Raymo
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May 24, 2019·The Journal of Physical Chemistry. B·Andrew RubaWeidong Yang
Nov 9, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Henry CoxJian R Lu

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