PMID: 9658097Jul 11, 1998Paper

Moloney murine leukemia virus envelope protein subunits, gp70 and Pr15E, form a stable disulfide-linked complex

Journal of Virology
D J OpsteltenHenrik Garoff

Abstract

The nature and stability of the interactions between the gp70 and Pr15E/p15E molecules of murine leukemia virus (MLV) have been disputed extensively. To resolve this controversy, we have performed quantitative biochemical analyses on gp70-Pr15E complexes formed after independent expression of the amphotropic and ecotropic Moloney MLV env genes in BHK-21 cells. We found that all cell-associated gp70 molecules are disulfide linked to Pr15E whereas only a small amount of free gp70 is released by the cells. The complexes were resistant to treatment with reducing agents in vivo, indicating that the presence and stability of the disulfide interaction between gp70 and Pr15E are not dependent on the cellular redox state. However, disulfide-bonded Env complexes were disrupted in lysates of nonalkylated cells in a time-, temperature-, and pH-dependent fashion. Disruption seemed not to be caused by a cellular factor but is probably due to a thiol-disulfide exchange reaction occurring within the Env complex after solubilization. The possibility that alkylating agents induce the formation of the intersubunit disulfide linkage was excluded by showing that disulfide-linked gp70-Pr15E complexes exist in freshly made lysates of nonalkylated cel...Continue Reading

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Citations

Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G L Beatty, Y Paterson
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
May 13, 2004·The Journal of Biological Chemistry·Su-Jung ParkSuk-Hee Lee
Nov 25, 2011·The Journal of General Virology·Masahiko ShinagawaHiroo Hoshino
Aug 14, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jamie E Henzy, Welkin E Johnson
May 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robin LövingHenrik Garoff
Apr 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mathilda SjöbergHenrik Garoff
Dec 21, 2007·Journal of Virology·Mathilda SjöbergHenrik Garoff
Feb 19, 2010·Biology of Reproduction·Andrej SusorMichal Kubelka
Feb 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mathilda SjöbergHenrik Garoff

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