PMID: 7537454Apr 1, 1995Paper

Moluccella laevis lectin, a marker for cellular differentiation programs in mouse gut epithelium

The American Journal of Physiology
P FalkJ I Gordon

Abstract

We have assembled a system for testing the hypothesis that changes in glycoconjugate production represent markers for defining developmental, spatial, and environmental influences on the proliferation and differentiation programs of various mouse gut epithelial cell lineages. Multilabel immunohistochemical methods were used to survey the interactions of purified lectins with 1) normal fetal, neonatal, and adult FVB/N mouse gut, 2) gastric and intestinal isografts harvested at various developmental stages, and 3) transgenic mouse models of intestinal epithelial cell hyperplasia, dysplasia, and/or neoplasia. As a demonstration of the system's utility, we used the recently purified, alpha-N-acetyl-D-galactosamine-specific, Moluccella laevis lectin (MLL). In the adult FVB/N mouse stomach, MLL only recognizes glycoconjugates produced by a population of nonproliferating neck and prezymogenic cells that occupy a pivotal point in the complex, migration-associated differentiation program of the zymogenic cell lineage. In the developing FVB/N stomach, MLL binds to members of the zymogenic and pit lineages even before morphogenesis of gastric units is completed. Expression of MLL epitopes in pit cells is restricted to the period before th...Continue Reading

Citations

Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Toshio NakajimaToshiyuki Takeuchi
Mar 19, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Faïza MaloumNathalie Perreault

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