MÖNCH detector enables fast and low-dose free-propagation phase-contrast computed tomography of in situ mouse lungs

Journal of Synchrotron Radiation
C DullinAnna Bergamaschi

Abstract

Due to the complexity of the underlying pathomechanism, in vivo mouse lung-disease models continue to be of great importance in preclinical respiratory research. Longitudinal studies following the cause of a disease or evaluating treatment efficacy are of particular interest but challenging due to the small size of the mouse lung and the fast breathing rate. Synchrotron-based in-line phase-contrast computed tomography imaging has been successfully applied in lung research in various applications, but mostly at dose levels that forbid longitudinal in vivo studies. Here, the novel charge-integrating hybrid detector MÖNCH is presented, which enables imaging of mouse lungs at a pixel size of 25 µm, in less than 10 s and with an entrance dose of about 70 mGy, which therefore will allow longitudinal lung disease studies to be performed in mouse models.

References

Oct 27, 2005·The British Journal of Radiology·M J KitchenK M Pavlov
Nov 7, 2007·Investigative Radiology·Soenke H BartlingFabian Kiessling
Mar 26, 2010·Radiation Research·Jacqueline P WilliamsWilliam H McBride
Sep 21, 2013·Journal of Applied Crystallography·Goran LovricRajmund Mokso
Mar 22, 2014·International Journal of Molecular Sciences·Eleonore Fröhlich, Sharareh Salar-Behzadi
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Oct 28, 2016·Journal of Synchrotron Radiation·Sebastian CartierMarco Stampanoni
Mar 7, 2017·Advanced Structural and Chemical Imaging·Francesco BrunAlessia Cedola

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Citations

Jul 4, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·J AlbersC Dullin
Feb 20, 2021·Scientific Reports·Amara KhanChristian Dullin
Oct 22, 2021·Tissue Engineering. Part C, Methods·Xiaoman DuanNing Zhu

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Methods Mentioned

BETA
X-ray
chip

Software Mentioned

STP
MÖNCH
SYRMEP
SCRY

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