Monitoring and control of Gluconacetobacter xylinus fed-batch cultures using in situ mid-IR spectroscopy

Journal of Biotechnology
Henri KornmannUrs von Stockar

Abstract

A partial least-squares calibration model, relating mid-infrared spectral features with fructose, ethanol, acetate, gluconacetan, phosphate and ammonium concentrations has been designed to monitor and control cultivations of Gluconacetobacter xylinus and production of gluconacetan, a food grade exopolysaccharide (EPS). Only synthetic solutions containing a mixture of the major components of culture media have been used to calibrate the spectrometer. A factorial design has been applied to determine the composition and concentration in the calibration matrix. This approach guarantees a complete and intelligent scan of the calibration space using only 55 standards. This calibration model allowed standard errors of validation (SEV) for fructose, ethanol, acetate, gluconacetan, ammonium and phosphate concentrations of 1.16 g/l, 0.36 g/l, 0.22 g/l, 1.54 g/l, 0.24 g/l and 0.18 g/l, respectively. With G. xylinus, ethanol is directly oxidized to acetate, which is subsequently metabolized to form biomass. However, residual ethanol in the culture medium prevents bacterial growth. On-line spectroscopic data were implemented in a closed-loop control strategy for fed-batch fermentation. Acetate concentration was controlled at a constant valu...Continue Reading

References

Jun 10, 1995·Analytical Biochemistry·U SchulzeJ Villadsen
Jul 21, 2001·Journal of Industrial Microbiology & Biotechnology·S SivakesavaA Demirci
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Maria G MacedoChristophe Lacroix
Apr 4, 2003·Biotechnology and Bioengineering·Henri KornmannUrs von Stockar
Apr 17, 2003·Applied Microbiology and Biotechnology·H KornmannU von Stockar

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Citations

May 31, 2012·Analytical and Bioanalytical Chemistry·N D LourençoH M Pinheiro
Jul 24, 2010·Applied Microbiology and Biotechnology·Daniel LandgrebeKenneth F Reardon
Oct 17, 2013·Microbial Cell Factories·Patrick SagmeisterChristoph Herwig
Dec 3, 2016·Analytical and Bioanalytical Chemistry·Jens ClaßenThomas Scheper
Aug 22, 2012·Lab on a Chip·Arkadiusz PierzchalskiAttila Tarnok
Feb 13, 2021·Trends in Biotechnology·Ivan SchlembachLars Regestein
Nov 27, 2019·New Biotechnology·Katrin PontiusMarta Bevilacqua

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