Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Royal Society Open Science
Yan DuKunlun Liu

Abstract

To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup ReadyTM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.

References

Aug 30, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shangxin SongHongmei Xiao
Nov 29, 2012·Applied Biochemistry and Biotechnology·Qingfeng GuanJianhua Wang
Oct 17, 2013·Food Chemistry·Zorica NikolićDragana Milošević
Nov 26, 2013·Journal of Nanoscience and Nanotechnology·Junli FengJishuang Chen
Mar 4, 2017·Food Chemistry·Junichi ManoKazumi Kitta
Dec 31, 2017·Food Chemistry·Zhengfei LuGary Swanson
Mar 16, 2018·Food Technology and Biotechnology·Zorica NikolićVuk Đorđević
Mar 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zora PiskataGabriela Borilova

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Datasets Mentioned

BETA
K00821
AB209952

Methods Mentioned

BETA
transgenic
PCR
electrophoresis
chemical treatments

Software Mentioned

QuantStudio TM Design & Analysis

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