Monitoring antigen-specific T cells using MHC-Ig dimers

Current Protocols in Immunology
Jonathan P SchneckTim F Greten

Abstract

The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.

Citations

Nov 2, 2016·Current Protocols in Immunology·John D Altman, Mark M Davis
May 12, 2021·Molecules and Cells·Jun Chang

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