Monitoring bacterial community shifts in bioleaching of Ni-Cu sulfide

Bioresource Technology
Zhiguo HeGuan-zhou Qiu

Abstract

The microbial ecology of the bioleaching of Ni-Cu sulfide is poorly understood and little effort has been made to handle the microbiological components of these processes. In this study, denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes fragments from bacteria was used to evaluate the changes of the bacterial community in the process of Ni-Cu sulfide bioleaching in a shaken flask system. The results revealed that the bacterial community was disturbed after the addition of Ni-Cu sulfide. Phylogenetic analyses of 16S rRNA fragments revealed that the retrieved sequences clustered together with the genera Acidithiobacillus and Leptospirillum. Multidimensional scaling (MDS) and cluster analysis of DGGE-banding patterns revealed that the process of Ni-Cu sulfide bioleaching in 46days was divided into three stages. During the bioleaching process of Ni-Cu sulfide, Leptospirillum was always dominant. The genera Acidithiobacillus was only detected at early and later stages of the bioleaching process. These results extend our knowledge on microbial dynamics in Ni-Cu sulfide bioleaching, a key issue required to improve commercial applications.

Citations

Dec 4, 2013·Journal of Hazardous Materials·Ewa KarwowskaAgnieszka Konarzewska
Jan 1, 2014·Bioresource Technology·Shuzhen LiGuohua Gu
Jun 3, 2011·Bioresource Technology·A P DasS Nayak
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Jan 11, 2020·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Alireza HabibiMalihe Hadadi

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