Monitoring bacterial indicators and pathogens in cattle feedlot waste by real-time PCR

Water Research
Marcus KleinD J Roser

Abstract

Quantitative microbial health risk assessment requires accurate enumeration of pathogens in hazard-containing matrices as part of the risk characterization process. As part of a risk management-oriented study of cattle feedlot waste contaminants, we investigated the utility of quantitative real-time PCR (qPCR) for surveying the microbial constituents of different faecal wastes. The abundance of Escherichia coli and enterococci were first estimated in five cattle feedlot waste types from five localities. Bacteria were quantified using two culture methods and compared to the number of genome copies detected by qPCR targeted at E. coli and Enterococcus faecalis. Bacterial numbers detected in the different wastes (fresh faeces, pen manure, aged manure, composted manure, carcass manure compost) ranged from 10-(7) to 10(2)g(-1) (dry weight). Both indicator groups were detected by qPCR with a comparable sensitivity to culture methods across this range. qPCR measurements of E. coli and E. faecalis correlated well with MPN and spread plate data. As a second comparison, we inoculated green fluorescent protein (GFP) labeled reference bacteria into manure samples. GFP labeled E. coli and Listeria monocytogenes were detected by qPCR in conc...Continue Reading

Citations

Dec 3, 2013·Journal of Environmental Management·Marieke ten HoeveSander Bruun
Aug 31, 2010·Applied and Environmental Microbiology·Marcus KleinDavid J Roser
Sep 18, 2012·Applied and Environmental Microbiology·Robert S DunganApril B Leytem
Oct 16, 2018·Journal of Food Protection·Joshua B GurtlerManan Sharma
May 16, 2013·Journal of Environmental Quality·A M P NetthisingheK R Sistani
Nov 13, 2013·Journal of Environmental Quality·Robert S Dungan, April B Leytem

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