Monitoring blood-flow in the mouse cochlea using an endoscopic laser speckle contrast imaging system

PloS One
Tae Hoon KongYoung Joon Seo

Abstract

Laser speckle contrast imaging (LSCI) enables continuous high-resolution assessment of microcirculation in real-time. We applied an endoscope to LSCI to measure cochlear blood-flow in an ischemia-reperfusion mouse model. We also explored whether using xenon light in combination with LSCI facilitates visualization of anatomical position. Based on a previous preliminary study, the appropriate wavelength for penetrating the thin bony cochlea was 830 nm. A 2.7-mm-diameter endoscope was used, as appropriate for the size of the mouse cochlea. Our endoscopic LSCI system was used to illuminate the right cochlea after dissection of the mouse. We observed changes in the speckle signals when we applied the endoscopic LSCI system to the ischemia-reperfusion mouse model. The anatomical structure of the mouse cochlea and surrounding structures were clearly visible using the xenon light. The speckle signal of the cochlea was scattered, with an intensity that varied between that of the stapes (with the lowest signal), the negative control, and the stapedial artery (with the highest signal), the positive control. In the cochlear ischemia-reperfusion mouse model, the speckle signal of the cochlea decreased during the ischemic phase, and increase...Continue Reading

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Citations

Aug 7, 2019·Journal of Biomedical Optics·Wido HeemanE Christiaan Boerma
May 9, 2019·Biomedical Optics Express·Corey ZhengJaepyeong Cha

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Methods Mentioned

BETA
dissection

Software Mentioned

SPSS
ARRIVE
Statistical Package for Social Sciences Software ( SPSS

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