Monitoring cell growth

Current Protocols in Immunology
W Strober

Abstract

This appendix provides two protocols for monitoring cell growth. Counting cells using a hemacytometer is tedious but it allows one to effectively distinguish live cells from dead cells (using Trypan Blue exclusion). In addition, this procedure is less subject to errors due to cell clumping or heterogeneity of cell size. The use of an electronic cell counter is quicker and easier than counting cells using a hemacytometer. However, an electronic cell counter as currently constructed does not distinguish live from dead cells in a reliable fashion and is subject to error due to the presence of cell clumps. Overall, the electronic cell counter is best reserved for repetitive and rapid counting of fresh peripheral blood cells and should be used with caution when counting cell populations derived from tissues.

Citations

Feb 13, 2016·Journal of the American Chemical Society·David PerryPatrick R Unwin
May 30, 2014·Scientific Reports·Mahmoud Al AhmadAli Hilal-Alnaqbi
Apr 4, 2017·Current Protocols in Immunology·Amrendra KumarSebastian Joyce
Aug 4, 2015·Current Protocols in Immunology·Yuzhi YinCalman Prussin
Feb 5, 2010·Current Protocols in Microbiology·Raquel HernandezDennis T Brown
Jun 22, 2012·Journal of Fluorescence·Leo L ChanNing Lai
Nov 4, 2015·Current Protocols in Immunology·Douglas B KuhnsKol A Zarember
May 8, 2020·Biological Procedures Online·Amin TavassoliHesam Dehghani
Feb 3, 2016·Current Protocols in Immunology·John T Schroeder, Anja P Bieneman
Apr 3, 2016·Current Protocols in Immunology·Gerritje J W van der WindtErika L Pearce
Nov 4, 2015·Current Protocols in Immunology·Warren Strober
Jul 4, 2018·Current Protocols in Immunology·Alexander MainiSimon Yona
Feb 7, 2017·Current Protocols in Microbiology·Asis Khan, Michael E Grigg
May 5, 2010·Current Protocols in Microbiology·Raquel Hernandez, Dennis T Brown
Aug 8, 2017·Cryobiology·Liana Monteiro da Fonseca CardosoLuiz Anastácio Alves

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