PMID: 8600434Jun 1, 1995Paper

Monitoring experimental alcelaphine herpesvirus-1 infection in cattle by nucleic-acid hybridization and PCR

The Onderstepoort Journal of Veterinary Research
A L MichelJ J Van der Lugt

Abstract

The DNA probe SW15 derived from the laboratory-attenuated Alcelaphine Herpesvirus-1 (AHV-1) strain WC11 as well as from the polymerase chain-reaction test (Hsu, Shih, Castro & Zee 1990), was used to detect viral DNA of malignant catarrhal fever (MCF) in six experimentally infected cattle. Heparinized blood samples were collected and tested at least three times a week over a period of up to 142 d. Results of hybridization and PCR tests were compared with the results of clinical examinations, and on various occasions with those of viral isolation and serum-neutralization assays as well as with those of pathology. Three animals developed clinical signs and lesions typical of MCF, while the other three animals remained clinically healthy. All cattle seroconverted, and viral nucleic acid was detected by DNA hybridization and PCR at various intervals during the observation period. Virus isolation was successful in two of the clinical cases and all cattle seroconverted. Storage of blood samples at 4 degrees C for up to 10 d did not influence the hybridization and DNA-amplification results.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.