Monitoring fibrinogen adsorption kinetics by interfacial TIRF rheometry

Journal of Molecular Recognition : JMR
A Sanders, H P Jennissen

Abstract

The adsorption kinetics of purified fibrinogen to unmodified and aminopropylsilane-modified quartz glass surfaces were studied under pseudo-first order (binding-unit excess) conditions by the total internal reflection fluorescence (TIRF) method. Fluorescence in the adsorbed protein layer (350 nm) was excited by the evanescent wave at 285-290 nm. In order to reduce and possibly eliminate the influence of mass transfer on the kinetics of fibrinogen adsorption, a novel protein adsorption chamber containing a cone-and-plate rheometer with total internal reflection technology was employed. The aim of the study was to obtain critical shear rates, at which the adsorption rate of fibrinogen became independent of diffusion. Therefore, shear rates were varied between 0 and 7200 s-1 at initial fibrinogen concentrations of c9 = 4.7 and 17.7 micrograms mL-1. The adsorption rate of fibrinogen increased 5-17-fold, depending on the surface, as the critical shear rate was approached. Above the critical shear rates the kinetic data of fibrinogen adsorption could be fitted at c9 = 4.7 micrograms mL-1 to a single exponential function, indicating the predominance of a single binding step with a half-life of ca 20 s. At the higher initial concentrat...Continue Reading

References

Sep 1, 1976·Scandinavian Journal of Clinical and Laboratory Investigation·N D Poe
Apr 24, 1992·Journal of Chromatography·H P Jennissen, A Demiroglou
Jul 1, 1993·The American Journal of Physiology·H J SchnittlerD Drenckhahn
Jul 1, 1951·Archives of Biochemistry and Biophysics·K LAKI
Jun 30, 1994·Colloids and Surfaces. B, Biointerfaces·Yeong-Shang Lin, Vladimir Hlady

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Citations

Mar 17, 2004·Biosensors & Bioelectronics·H P Jennissen, T Zumbrink
Jan 27, 2012·Chemical Reviews·Francisco Zaera
Feb 16, 2017·Eye & Contact Lens·Brad HallLyndon Jones
Jun 14, 2018·Journal of Biomedical Materials Research. Part a·Thomas A Horbett

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