PMID: 7540919Jan 1, 1995Paper

Monitoring for engraftment following rat orthotopic liver transplantation by in vitro amplification of Y-chromosome gene using polymerase chain reaction

Cell Transplantation
H TashiroK Dohi

Abstract

The polymerase chain reaction (PCR) using primers specific for the rat Y-chromosome gene made it possible to distinguish a very small number of male rat cells from a large excess of female rat cells. In nonimmunosuppressed LEW recipients of ACI liver allografts, the donor cells in the bloodstream disappeared rapidly by day 3, earlier than the biochemical changes indicative of liver dysfunction. In immunosuppressed LEW recipients of ACI liver allografts, the donor cells were detected for a longer time. Moreover, in LEW recipients surviving for long period, the PCR revealed mixed-microchimerism. Our results indicated that this Y chromosomal gene-specific PCR method is useful for assessing engraftment following rat liver transplantation.

Citations

Aug 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Keiichi Muramatsu, Allen T Bishop
Feb 1, 2003·Plastic and Reconstructive Surgery·Keiichi MuramatsuRichard G Valenzuela
May 1, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M SiemionowK Carnevale
Feb 10, 2009·The Journal of Surgical Research·Keiichi MuramatsuToshihiko Taguchi

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