Monitoring granule formation in anaerobic upflow bioreactors using oligonucleotide hybridization probes

Biotechnology and Bioengineering
Dandan ZhengL Raskin

Abstract

The process of granule formation in upflow anaerobic sludge blanket (UASB) reactors was studied using oligonucleotide hybridization probes. Two laboratory-scale UASB reactors were inoculated with sieved primary anaerobic digester sludge from a municipal wastewater treatment plant and operated similarly except that reactor G was fed glucose, while reactor GP was fed glucose and propionate. Size measurements of cell aggregates and quantification of different populations of methanogens with membrane hybridization targeting the small-subunit ribosomal RNA demonstrated that the increase in aggregate size was associated with an increase in the abundance of Methanosaeta concilii in both reactors. In addition, fluorescence in situ hybridization showed that the major cell components of small aggregates collected during the early stages of reactor startup were M. concilii cells. These results indicate that M. concilii filaments act as nuclei for granular development. The increase in aggregate size was greater in reactor GP than in reactor G during the early stages of startup, suggesting that the presence of propionate-oxidizing syntrophic consortia assisted the formation of granules. The mature granules formed in both reactors exhibited ...Continue Reading

References

Sep 1, 1992·Applied and Environmental Microbiology·R I AmannK H Schleifer
Jul 1, 1991·Applied and Environmental Microbiology·J T GrotenhuisA J Zehnder
Jun 1, 1990·Applied and Environmental Microbiology·F A MacLeodJ W Costerton
Jan 1, 1980·Archives of Microbiology·A J ZehnderK Wuhrmann
Apr 1, 1994·Applied and Environmental Microbiology·L RaskinD A Stahl
Jan 1, 1995·Antonie van Leeuwenhoek·G Lettinga
Sep 30, 2000·Applied and Environmental Microbiology·E W AlmL Raskin
Mar 11, 2004·Environmental Microbiology·Largus T AngenentLutgarde Raskin
Mar 1, 1992·Applied and Environmental Microbiology·J T GrotenhuisA J Zehnder
Jan 1, 1996·Applied and Environmental Microbiology·L RaskinD A Stahl
Jun 1, 1996·Applied and Environmental Microbiology·W WuJ G Zeikus
Nov 1, 1985·Biotechnology and Bioengineering·W M Wiegant, G Lettinga
Feb 5, 1996·Biotechnology and Bioengineering·J E Schmidt, B K Ahring

❮ Previous
Next ❯

Citations

Sep 30, 2008·Applied and Environmental Microbiology·Xu LiLutgarde Raskin
Jul 13, 2012·BMC Microbiology·Nils Johan FredrikssonBritt-Marie Wilén
Sep 12, 2013·International Journal of Molecular Sciences·Kadiya CalderónJesús González-López
Jul 21, 2007·Water Research·Toshio ShimadaEberhard Morgenroth
Aug 19, 2007·Journal of Bioscience and Bioengineering·Daisuke Baba, Arata Katayama
Jul 31, 2007·Bioresource Technology·B MonteroR Solera
Apr 28, 2007·Current Opinion in Biotechnology·Takashi Narihiro, Yuji Sekiguchi
Sep 11, 2010·Biotechnology and Bioengineering·Toshio ShimadaLutgarde Raskin
Sep 19, 2015·Applied Microbiology and Biotechnology·Zhiji DingGiovanni Esposito
Apr 20, 2016·Journal of Environmental Management·Mateus PirolliHugo Moreira Soares
Aug 2, 2017·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Lihua NiuQing Wang
Mar 17, 2020·Frontiers in Microbiology·Maria Cristina GaglianoCaroline M Plugge
Feb 9, 2021·Frontiers in Microbiology·Patricia Bovio-WinklerClaudia Etchebehere
Jan 11, 2020·New Biotechnology·Maria Cristina GaglianoCaroline M Plugge
Nov 7, 2021·Bulletin of Mathematical Biology·A TenoreL Frunzo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.