Monitoring heart function in larval Drosophila melanogaster for physiological studies.

Journal of Visualized Experiments : JoVE
Ann S CooperRobin L Cooper

Abstract

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained and restrained whole larvae. For direct control of the environment around the heart another approach utilizes the dissected larvae and removal of the internal organs in order to bathe the heart in desired compounds. The exposed heart also allows membrane potentials to be monitored which can give insight of the ionic currents generated by the myocytes and for electrical conduction along the heart tube. These approaches have various advantages and disadvantages for future experiments that are discussed. The larval heart preparation provides an additional model besides the Drosophila skeletal NMJ to investigate the role of intracellular calcium regulation on cellular function. Learning more about the underlying ionic currents that shape the action potentials in myocytes in various species, one can hope to get a handle on the known ionic dysfunctions associated to specific genes responsible for various diseases in mammals.

References

Nov 1, 1995·Journal of Neurobiology·G G Gu, S Singh
Dec 1, 1995·Journal of Neurogenetics·H DowseL White
Feb 1, 1997·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·E JohnsonH Dowse
Jul 1, 1997·General Pharmacology·T A Miller
Nov 26, 1999·Peptides·R NicholsE Zornik
Mar 18, 2000·Kidney International·B Ganetzky
Jun 19, 2001·Mechanisms of Development·B He, P N Adler
Sep 28, 2001·In Vitro Cellular & Developmental Biology. Animal·C Papaefthmiou, G Theophilidis
Mar 29, 2002·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·E JohnsonH Dowse
Nov 6, 2004·Gene·Ethan Bier, Rolf Bodmer
Jan 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Davide Dulcis, Richard B Levine
Mar 29, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nicolas H BadreRobin L Cooper
May 17, 2005·Journal of Insect Physiology·Karel Sláma, Robert Farkas
Dec 20, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Sameera Dasari, Robin L Cooper
Nov 28, 2006·Mechanisms of Ageing and Development·Karen OcorrRolf Bodmer
Jul 12, 2007·PloS One·Karen A OcorrRolf Bodmer
Jul 26, 2007·The European Journal of Neuroscience·Sameera DasariRobin L Cooper
May 12, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Samantha PeronAram Megighian

❮ Previous
Next ❯

Citations

Mar 19, 2011·Pharmacological Reviews·Udai Bhan Pandey, Charles D Nichols
Dec 12, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Wendi S Neckameyer, Kathryn J Argue
Dec 29, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Zana R MajeedRobin L Cooper
Nov 6, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Clara de CastroRobin L Cooper
Oct 7, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Cole A MalloyRobin L Cooper
Aug 2, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Zana R MajeedRobin L Cooper
May 23, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yue Chen ZhuRobin L Cooper
Mar 5, 2016·Disease Models & Mechanisms·Berrak UgurHugo J Bellen
Mar 5, 2016·Disease Models & Mechanisms·Matthew J Moulton, Anthea Letsou
Aug 25, 2019·Methods and Protocols·Catherine E StanleyRobin L Cooper
Jun 1, 2020·Invertebrate Neuroscience : in·Catherine E StanleyRobin L Cooper
Jan 23, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Alireza ZabihihesariPouya Rezai
Dec 5, 2013·Physiological Reports·Josh S TitlowRobin L Cooper

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoreses
dissection
Dissect

Related Concepts

Related Feeds

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.

Related Papers

Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
Clara de CastroRobin L Cooper
Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Sameera Dasari, Robin L Cooper
Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Björn Lemmer
© 2022 Meta ULC. All rights reserved