Monitoring in real time with a microelectrode the release of reactive oxygen and nitrogen species by a single macrophage stimulated by its membrane mechanical depolarization

Chembiochem : a European Journal of Chemical Biology
Christian AmatoreYuehong Tong

Abstract

Macrophages are key cells of the immune system. During phagocytosis, the macrophage engulfs a foreign bacterium, virus, or particle into a vacuole, the phagosome, wherein oxidants are produced to neutralize and decompose the threatening element. These oxidants derive from in situ production of superoxide and nitric oxide by specific enzymes. However, the chemical nature and sequence of release of these compounds is far from being completely determined. The aim of the present work was to study the fundamental mechanism of oxidant release by macrophages at the level of a single cell, in real time and quantitatively. The tip of a microelectrode was positioned at a micrometric distance from a macrophage in a culture to measure oxidative-burst release by the cell when it was submitted to physical stimulation. The ensuing release of electroactive reactive oxygen and nitrogen species was detected by amperometry and the exact nature of the compounds was characterized through comparison with in vitro electrochemical oxidation of H2O2, ONOO-, NO*, and NO2(-) solutions. These results enabled the calculation of time variations of emission flux for each species and the reconstruction of the original flux of production of primary species, O2...Continue Reading

References

Jan 1, 1992·Free Radical Biology & Medicine·D W KampS A Weitzman
Dec 11, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M WightmanO H Viveros
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·L HuangR T Kennedy
Jun 30, 1993·Biochemical and Biophysical Research Communications·T MalinskiP Tomboulian
Jul 12, 1993·FEBS Letters·P C FordD M Stanbury
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M E de VeraD A Geller
Oct 1, 1995·Analytical Chemistry·S ArbaultC Amatore
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·G García-CardeñaW C Sessa
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y XiaJ L Zweier
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L CastilloV R Young
Jun 20, 1997·The Journal of Biological Chemistry·J B MichelT Michel
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y Xia, J L Zweier
Nov 14, 1997·Physiological Reviews·A B Parekh, R Penner
Apr 8, 1998·The American Journal of Physiology·P R MilesL Huffman
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·E R Travis, R M Wightman
Jul 22, 1998·Chemical Research in Toxicology·W H Koppenol
Jul 24, 1998·Biophysical Journal·B ChenW M Deen
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Vásquez-VivarK A Pritchard
Jan 1, 1997·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S ArbaultM Vuillaume
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·T E DeCourseyL L Thomas
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·C Nathan, M U Shiloh
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·D M CannonA G Ewing
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·M L HamiltonA Richardson
Mar 20, 2002·Nature Cell Biology·Anke DiDeborah J Nelson
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Andrei L Kindzelskii, Howard R Petty
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael G EspeyDavid A Wink
Oct 17, 2002·Analytical Chemistry·Tomoyuki YasukawaTomokazu Matsue
Oct 31, 2002·Immunologic Research·Karen E Iles, Henry Jay Forman
Nov 14, 2002·Pharmacological Reviews·Illarion V Turko, Ferid Murad
Mar 7, 2003·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian AmatoreManon Guille
Jun 28, 2003·Journal of Cell Science·Jennifer L Bankers-FulbrightScott M O'Grady
Oct 22, 2003·Circulation·Kathy K Griendling, Garret A FitzGerald
May 26, 2004·Journal of Cell Science·William C Sessa
Jun 1, 2004·Journal of Cell Science·Imran N Mungrue, David S Bredt

❮ Previous
Next ❯

Citations

Mar 28, 2008·Analytical and Bioanalytical Chemistry·Jean-Francois MassonChristine Kranz
Mar 13, 2009·Analytical and Bioanalytical Chemistry·Sabine Borgmann
May 12, 2009·Analytical and Bioanalytical Chemistry·Michaela PekarovaJan Hrbac
Aug 1, 2009·Journal of Materials Science. Materials in Medicine·Masayuki TairaYoshima Araki
Jan 5, 2013·Analytical Chemistry·Rebecca A HunterMark H Schoenfisch
Jun 26, 2008·Chemical Reviews·Donita L RobinsonR Mark Wightman
Jun 5, 2013·The Analyst·Raluca OpreaSabine Szunerits
Jan 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peng SunMichael V Mirkin
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yixian WangChristian Amatore
Jan 1, 2014·ACS Nano·Paolo ActisYuri E Korchev
Mar 13, 2014·Physical Chemistry Chemical Physics : PCCP·Roberto GiacovazziCarlo Adamo
Apr 6, 2012·Journal of Fluorescence·Eliana F C SimõesJoaquim C G Esteves da Silva
Mar 13, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Anne-Sophie BernardClotilde Policar
Dec 27, 2011·Bioelectrochemistry·Raluca MarcuMarco Giorgio
Sep 21, 2011·Cell Calcium·Ivan BogeskiBarbara A Niemeyer
Aug 22, 2009·Small·Iddo HellerCees Dekker
Oct 17, 2008·Angewandte Chemie·Milos R FilipovićIvana Ivanović-Burmazović
Oct 20, 2007·Angewandte Chemie·Albert Schulte, Wolfgang Schuhmann
May 21, 2008·Chembiochem : a European Journal of Chemical Biology·Christian AmatoreAlaric C W Koh
May 2, 2015·The New Phytologist·Aysin Guzel DegerM Rob G Roelfsema
Jan 20, 2012·Biosensors & Bioelectronics·Leslie A HiattDavid E Cliffel
Jul 28, 2007·Biochemical and Biophysical Research Communications·Yann VerchierChristian Amatore
Jul 1, 2008·Annual Review of Analytical Chemistry·Kelly L AdamsAndrew G Ewing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.