Monitoring intrahepatic CD8+ T cells by fine-needle aspiration cytology in chronic hepatitis C infection

Journal of Viral Hepatitis
J M VrolijkSolko W Schalm

Abstract

Infection of the liver with hepatitis C virus (HCV) causes compartmentalization of CD8+ cytotoxic T cells to the site of disease. These cells are thought to be involved in viral clearance during interferon therapy. The repetitive analysis of the intrahepatic immune response is hampered by the difficulty to obtain the intrahepatic T cells. The fine-needle aspiration biopsy (FNAB) technique was evaluated for its use to obtain liver-derived CD8+ T cells in a minimally invasive way. In 26 chronic HCV patients who were evaluated for Peg-interferon and ribavirin combination therapy, pre-treatment FNABs and peripheral blood specimens were obtained simultaneously with liver tissue biopsies, and CD3+ and CD8+ T cells were quantified by immunocytochemistry. The CD8+/CD3+ ratio was significantly higher in the FNABs than in peripheral blood (P < 0.01), and similar to those in portal areas in the tissue biopsies. A significant correlation was observed between numbers of CD3+CD8+ T lymphocytes in the FNABs and the numbers of CD8+ cells in the lobular fields or in the portal tracts of the liver tissue biopsies, but not with CD3+CD8+ T lymphocytes in peripheral blood. Finally, the ratio of CD8+/CD3+ T lymphocytes in FNABs was significantly hig...Continue Reading

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Citations

Aug 1, 2007·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·James D Perkins
Dec 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jason T BlackardKenneth E Sherman
Jan 31, 2014·Journal of Viral Hepatitis·S LejnineA H Talal
Jan 4, 2006·International Journal of Immunopathology and Pharmacology·R CianciF Pandolfi

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