Monitoring microevolution of OXA-48-producing Klebsiella pneumoniae ST147 in a hospital setting by SMRT sequencing

The Journal of Antimicrobial Chemotherapy
Andreas E ZautnerWolfgang Bohne

Abstract

Carbapenemase-producing Klebsiella pneumoniae pose an increasing risk for healthcare facilities worldwide. A continuous monitoring of ST distribution and its association with resistance and virulence genes is required for early detection of successful K. pneumoniae lineages. In this study, we used WGS to characterize MDR blaOXA-48-positive K. pneumoniae isolated from inpatients at the University Medical Center Göttingen, Germany, between March 2013 and August 2014. Closed genomes for 16 isolates of carbapenemase-producing K. pneumoniae were generated by single molecule real-time technology using the PacBio RSII platform. Eight of the 16 isolates showed identical XbaI macrorestriction patterns and shared the same MLST, ST147. The eight ST147 isolates differed by only 1-25 SNPs of their core genome, indicating a clonal origin. Most of the eight ST147 isolates carried four plasmids with sizes of 246.8, 96.1, 63.6 and 61.0 kb and a novel linear plasmid prophage, named pKO2, of 54.6 kb. The blaOXA-48 gene was located on a 63.6 kb IncL plasmid and is part of composite transposon Tn1999.2. The ST147 isolates expressed the yersinabactin system as a major virulence factor. The comparative whole-genome analysis revealed several rearrange...Continue Reading

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Citations

Jun 27, 2019·International Journal of Molecular Sciences·Cheng ZhangFengjie Sun
Nov 15, 2019·Clinical Microbiology Reviews·Johann D D PitoutYasufumi Matsumura
May 18, 2020·The Journal of Antimicrobial Chemotherapy·Jennifer MoussaSima Tokajian
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Jul 21, 2021·Journal of Global Antimicrobial Resistance·Lifeng WangJiyong Yang
Nov 7, 2021·The Science of the Total Environment·Katja KehlGabriele Bierbaum

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